Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon.
Lectin binding and gel secretion within Lorenzinian electroreceptors of Polyodon.
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DOI：
10.1371/journal.pone.0276854
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发表时间：
2022
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影响因子：
3.7
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中科院分区：
文献类型：
--
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我们成像的碳水化合物选择性结合的8个凝集素在壶腹器官（AO）的电感受器的嘴部淡水白鲟（Polyodon spathula），通过荧光成像和冰冻切片的形态计量学。一个重点是候选网站的分泌糖蛋白凝胶填充管腔的AO。多齿龙的喙是头部前方的一个电感觉附属物，覆盖有超过50，000个AO，每个AO与海洋鳐和鲨鱼的Lorenzini电感受器壶腹同源。一个大的电感觉神经上皮（EN）内衬每个AO的腔底极在Polyodon;支持细胞占据大部分（97%）EN的顶端区域，沿着与电敏感受体细胞。(1)凝集素WGA或SBA标记AO凝胶。高浓度的N-乙酰基-氨基碳水化合物的配体，这些凝集素的报告在管凝胶的壶腹的Lorenzini，支持Polyodon AO的同源性。在EN的横切面上，WGA或SBA标记支持细胞的胞质小泡和细胞器，特别是顶端，明显分泌。支持细胞顶面上丰富的鬼笔环肽+微绒毛产生最亮的凝集素WGA或SBA标记。在平行视图的顶端EN表面，WGA标记的支持细胞。我们得出结论，EN支持细胞从其顶端微绒毛（和表面？）大量分泌凝胶，含有WGA或SBA的氨基碳水化合物配体，进入AO腔。(2)凝集素RCA 120或ConA也标记EN支持细胞，各自不同。RCA 120-荧光素明亮地标记广泛的高尔基体小管在顶端的EN细胞的一半。刀豆蛋白A没有标记微绒毛，但明亮地标记整个支持细胞的小泡，显然非分泌。(3)我们证明了“插座”周围的EN受体细胞的基底外侧的外部，作为候选糖萼。(4)我们探讨了是否额外的分泌物可能来自壶腹内壁的非EN上皮细胞。(5)模型：凝胶主要由覆盖每个AO基底极的大EN中的支持细胞分泌。分泌的凝胶被推向毛孔，然后排出。由于壶腹壁的几何锥度，我们将凝胶速度建模为增加~ 11倍，在AO中向远端（朝向狭窄的颈部和孔）。凝胶更新和加速排出可以防御微生物或小寄生虫对AO腔的入侵。(6)我们调查凝集素标记的附属结构，包括在AO颈部的乳头细胞，横纹外胚层表皮，鞘传入轴突或终端胶质细胞。
We imaged the carbohydrate-selective spatial binding of 8 lectins in the ampullary organs (AOs) of electroreceptors on the rostrum of freshwater paddlefish (Polyodon spathula), by fluorescence imaging and morphometry of frozen sections. A focus was candidate sites of secretion of the glycoprotein gel filling the lumen of AOs. The rostrum of Polyodon is an electrosensory appendage anterior of the head, covered with >50,000 AOs, each homologous with the ampulla of Lorenzini electroreceptors of marine rays and sharks. A large electrosensory neuroepithelium (EN) lines the basal pole of each AO’s lumen in Polyodon; support cells occupy most (97%) of an EN’s apical area, along with electrosensitive receptor cells. (1) Lectins WGA or SBA labeled the AO gel. High concentrations of the N-acetyl-aminocarbohydrate ligands of these lectins were reported in canal gel of ampullae of Lorenzini, supporting homology of Polyodon AOs. In cross sections of EN, WGA or SBA labeled cytoplasmic vesicles and organelles in support cells, especially apically, apparently secretory. Abundant phalloidin+ microvilli on the apical faces of support cells yielded the brightest label by lectins WGA or SBA. In parallel views of the apical EN surface, WGA labeled only support cells. We concluded that EN support cells massively secrete gel from their apical microvilli (and surface?), containing amino carbohydrate ligands of WGA or SBA, into the AO lumen. (2) Lectins RCA120 or ConA also labeled EN support cells, each differently. RCA120-fluorescein brightly labeled extensive Golgi tubules in the apical halves of EN cells. ConA did not label microvilli, but brightly labeled small vesicles throughout support cells, apparently non-secretory. (3) We demonstrated “sockets” surrounding the basolateral exteriors of EN receptor cells, as candidate glycocalyces. (4) We explored whether additional secretions may arise from non-EN epithelial cells of the interior ampulla wall. (5) Model: Gel is secreted mainly by support cells in the large EN covering each AO’s basal pole. Secreted gel is pushed toward the pore, and out. We modeled gel velocity as increasing ~11x, going distally in AOs (toward the narrowed neck and pore), due to geometrical taper of the ampulla wall. Gel renewal and accelerated expulsion may defend against invasion of the AO lumen by microbes or small parasites. (6) We surveyed lectin labeling of accessory structures, including papilla cells in AO necks, striated ectoderm epidermis, and sheaths on afferent axons or on terminal glia.
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DOI：
10.1083/jcb.200510043
发表时间：
2005-12-19
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ikenouchi J;Furuse M;Furuse K;Sasaki H;Tsukita S;Tsukita S
通讯作者：
Tsukita S
DOI：
10.1242/jeb.082362
发表时间：
2013-07-01
期刊：
The Journal of experimental biology
影响因子：
--
作者：
Baker CV;Modrell MS;Gillis JA
通讯作者：
Gillis JA
影响因子：
4.1
作者：
DOYLE, J
通讯作者：
DOYLE, J
DOI：
10.1007/bf00338931
发表时间：
1962-01-01
期刊：
ZEITSCHRIFT FUR ZELLFORSCHUNG UND MIKROSKOPISCHE ANATOMIE
影响因子：
--
作者：
KANTNER, M;KONIG, WF;REINBACH, W
通讯作者：
REINBACH, W
影响因子：
7.8
作者：
ETZLER, ME;BRANSTRATOR, ML
通讯作者：
BRANSTRATOR, ML