The effect of allogeneic cardiac stem cells in left ventricular geometry and function in a rat model of myocardial infarction
The effect of allogeneic cardiac stem cells in left ventricular geometry and function in a rat model of myocardial infarction
复制标题
同种异体心脏干细胞对心肌梗死大鼠模型左心室几何结构和功能的影响
DOI：
10.1111/1755-5922.12313
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发表时间：
2018-02
期刊：
影响因子：
--
通讯作者：
Wang Tong
中科院分区：
文献类型：
--
作者：
Weng Yinlun;Zhang Xueyuan;Liu Anmin;Cai Wangqing;Li Fangcheng;Wang Tong
背景
急性心肌梗死（MI）后心力衰竭的发生与左室（LV）病理性重构和功能障碍有关。心脏干细胞为急性心肌梗死的治疗提供了新的选择。本研究旨在探讨CSCs对急性心肌梗死心肌结构和功能改变的影响。
方法
通过结扎冠状动脉左前降支在20只Sprague-Dawley大鼠中诱导急性MI。MI后2周，将动物随机分为CSC组和对照组。在基线、MI后2周、4周和6周通过超声心动图测量LV几何形状和功能。于MI后6周测定血流动力学指标，取心肌行PKH 26染色，RT-PCR和ELISA检测心肌细胞VEGF-α/TGF-β1的表达。
结果
MI后2周，室间隔收缩期和舒张期厚度（IVSTs/d）明显减小，而左室收缩期和舒张期内径（LVDs/d）明显增大。因此，这导致射血分数（EF）和缩短分数（FS）降低。CSCs治疗4周后，CSCs组射血分数（EF）和短缩分数（FS）明显改善，左室收缩期缩短，左室舒张末期内径（LVDs/d）明显减小。此外，观察到LV压力发展和下降的最大速率（分别为峰值+dP/dt和-dP/dt）显著改善。此外，CSC组VEGF-α的表达明显高于TGF-β1。
结论
心肌梗死后CSCs表达VEGF-α增加，可显著防止心肌结构和功能的恶化。
BACKGROUND
The development of heart failure after acute myocardial infarction (MI) was related to left ventricular (LV) pathological remodeling and dysfunction. Cardiac stem cells (CSCs) provided a new option to treat acute MI. This study was to investigate the effects of CSCs on structural and functional alteration in acute MI.
METHODS
Acute MI was induced in 20 Sprague-Dawley rats by ligation of the left anterior descending coronary artery. Two weeks after MI, animals were randomized into CSCs or control group. LV geometry and function were echocardiographically measured at baseline, 2, 4, and 6 weeks after MI. After measuring hemodynamics at 6 weeks after MI, hearts were harvested for tracing CSCs stained by PKH26 and testing expression of VEGF-α/TGF-β1 by RT-PCR and ELISA.
RESULTS
Two weeks after MI, there were significant decreases in interventricular septal systolic and diastolic thickness (IVSTs/d), while increases in LV systolic and diastolic dimension (LVDs/d). Consequently, this contributed to decreases in ejection fraction (EF) and fractional shorting (FS). With the treatment of CSCs for 4 weeks, significant better ejection fraction (EF) and fractional shorting (FS) were achieved in CSCs group accompanied by the reduction in LV systole and diastole dimension (LVDs/d). Besides, a significant improvement in the maximal rate of LV pressure development and decline (peak +dP/dt and -dP/dt, respectively) was observed. Moreover, significantly higher VEGF-α was expressed in CSCs group rather than TGF-β1.
CONCLUSION
CSCs significantly prevented structural and functional deterioration after MI with increasing expression of VEGF-α.
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影响因子：
64.8
作者：
K. Laugwitz;A. Moretti;J. Lam;P. Gruber;Y. Chen;S. Woodard;L. Lin;C. Cai;M. Lu;M. Reth-M.-R
通讯作者：
K. Laugwitz;A. Moretti;J. Lam;P. Gruber;Y. Chen;S. Woodard;L. Lin;C. Cai;M. Lu;M. Reth-M.-R
影响因子：
--
作者：
Dong-feng Lu;Hao Wu;Jing-Shuang Huang;Yan Li
通讯作者：
Dong-feng Lu;Hao Wu;Jing-Shuang Huang;Yan Li
影响因子：
8.8
作者：
Tong Wang;Wanchun Tang;Shijie Sun;G. Ristagno;Zitong Huang;M. Weil
通讯作者：
Tong Wang;Wanchun Tang;Shijie Sun;G. Ristagno;Zitong Huang;M. Weil
影响因子：
64.8
作者：
Laugwitz, KL;Moretti, A;Chien, KR
通讯作者：
Chien, KR
影响因子：
37.8
作者：
HELAK, JW;REICHEK, N
通讯作者：
REICHEK, N