Glycolytic regulation in aestivation of the sea cucumber Apostichopus japonicus: evidence from metabolite quantification and rate-limiting enzyme analyses
Glycolytic regulation in aestivation of the sea cucumber Apostichopus japonicus: evidence from metabolite quantification and rate-limiting enzyme analyses
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海参夏眠中的糖酵解调节：来自代谢物定量和限速酶分析的证据
DOI：
10.1007/s00227-016-2936-5
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发表时间：
2016-07
期刊：
影响因子：
2.4
通讯作者：
Wang Tianming
中科院分区：
文献类型：
--
作者：
Xiang Xiaowei;Chen Muyan;Wu Changwen;Zhu Aiyi;Yang Jingwen;Lv Zhenming;Wang Tianming
休眠是各种动物的一种休眠状态，其特征是新陈代谢受阻。夏季的高温会引发成体刺参的夏眠。在本研究中，通过代谢产物的定量和活性以及限速酶(己糖和丙酮酸激酶)的基因表达分析，证实了夏眠日本对虾的糖酵解抑制作用。日本对虾的呼吸树、肠道和肌肉中的葡萄糖浓度显著降低；同样，在此期间，呼吸树和肠道中的丙酮酸浓度显著下降，但肌肉中的丙酮酸浓度没有变化。己糖酶(AJ-HK)和丙酮酸酶(AJ-PK)在日本对虾的解冻过程中的活性变化表明，AJ-HK在呼吸树和肌肉中保持较高水平，但在肠道中显著下降(P<0.01)，而在呼吸树(P<0.05)和肌肉(P<0.01)中活性显著升高。对AJ-HK和AJ-PK的基因序列进行了鉴定，转录定量结果显示AJ-HK在所有受试组织中表达下调，而AJ-PK仅在肌肉中表达下调。这些结果表明，在夏眠的日本对虾中，糖酵解抑制发生了，尤其是在肠道中，转录和转录后修饰参与了对这种海洋物种糖酵解抑制的调节。
Aestivation is a state of dormancy in diverse animal species characterized by a depressed metabolism. High temperatures in the summer trigger aestivation in the adult sea cucumber, Apostichopus japonicus. In the present study, the glycolytic suppression in aestivating A. japonicus was verified by metabolite quantification and activity and a gene expression analysis of rate-limiting enzymes (hexokinase and pyruvate kinase). Significant decreases in glucose concentrations were discovered in the respiration tree (p < 0.01), intestine (p < 0.05) and muscle (p < 0.01) of aestivating A. japonicus; likewise, over this period, pyruvic acid concentrations significantly decreased in the respiration tree (p < 0.05) and intestine (p < 0.01), but were unchanged in muscle. Enzymatic activity variation of hexokinase (Aj-HK) and pyruvate kinase (Aj-PK) in aestivating A. japonicus revealed that Aj-HK remained at high levels in the respiration tree and muscle but significantly decreased in intestine (p < 0.01); the activity of Aj-PK also declined significantly in intestine (p < 0.01), whereas it increased significantly in the respiration tree (p < 0.05) and muscle (p < 0.01). The gene sequences of Aj-HK and Aj-PK were identified, and their transcriptional quantification revealed that the expression of Aj-HK was depressed in all of the examined tissues, whereas Aj-PK expression was depressed in muscle only. These results suggest that glycolytic suppression occurred in aestivating A. japonicus, especially in the intestine, and transcriptional and post-transcriptional modifications are involved in the regulation of glycolytic suppression in this marine species.
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影响因子：
--
作者：
Bell RA;Dawson NJ;Storey KB
通讯作者：
Storey KB
影响因子：
1.9
作者：
J. A. Stuart;T. Gillis;J. Ballantyne
通讯作者：
J. A. Stuart;T. Gillis;J. Ballantyne
影响因子：
--
作者：
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DOI：
10.1016/j.cbpa.2009.09.001
发表时间：
2009-12-01
影响因子：
2.3
作者：
Kayes, Sara M.;Cramp, Rebecca L.;Franklin, Craig E.
通讯作者：
Franklin, Craig E.
DOI：
10.1007/s00360-011-0613-z
发表时间：
2012-02-01
影响因子：
2
作者：
Loong, A. M.;Hiong, K. C.;Ip, Y. K.
通讯作者：
Ip, Y. K.