Comprehensive analysis of immune-related biomarkers and pathways in intracerebral hemorrhage using weighted gene co-expression network analysis and competing endogenous ribonucleic acid.
Comprehensive analysis of immune-related biomarkers and pathways in intracerebral hemorrhage using weighted gene co-expression network analysis and competing endogenous ribonucleic acid.
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DOI：
10.3389/fnmol.2022.955818
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发表时间：
2022
影响因子：
4.8
通讯作者：
He, Zhiyi
中科院分区：
文献类型：
--
作者：
Hao, Yuehan;Xu, Xiaoxue;Wang, Yuye;Jin, Feng;Tang, Ling;Zheng, Wenxu;Zhang, Heyu;He, Zhiyi
免疫反应是脑出血后继发性脑损伤的重要组成部分，与神经功能缺失及预后密切相关。免疫反应和炎症反应的机制对脑损伤和潜在的功能恢复具有重要意义；然而，脑出血患者外周血中免疫相关的生物标志物和竞争的内源性核糖核酸(CERNA)网络尚未构建。我们采集了脑出血患者和对照组的外周血，用LC Human Cerna芯片检测它们的CENA表达谱，并用qRT-PCR验证它们的表达。在脑出血患者CERNA芯片中检测到211个DElncRNAs和101个DEmRNAs。功能浓缩分析结果表明，免疫反应是脑出血病理过程中的重要组成部分。结合加权基因共表达网络分析(WGCNA)，我们构建的免疫相关CERNA网络中存在12个lncRNAs、10个miRNAs和7个mRNAs。本研究首次建立了WGCNA来源的免疫相关ceRNAs的网络，并确定白血病抑制因子(LIF)和B细胞淋巴瘤样13(BCL2L13)是脑出血患者外周血中关键的免疫相关生物标志物，它们可能与PI3K-Akt、MAPK信号通路和氧化磷酸化相关。MOXD2P-miR-211-3p-LIF和LINC00299-miR-198-BCL2L13轴可能参与了脑出血的免疫调节机制。我们研究的目的是为脑出血的潜在免疫调节机制提供创新的见解，并确定可能的免疫干预靶点。
The immune response is an important part of secondary brain injury following intracerebral hemorrhage (ICH), and is related to neurological deficits and prognosis. The mechanisms underlying the immune response and inflammation are of great significance for brain injury and potential functional restoration; however, the immune-related biomarkers and competing endogenous ribonucleic acid (RNA) (ceRNA) networks in the peripheral blood of ICH patients have not yet been constructed. We collected the peripheral blood from ICH patients and controls to assess their ceRNA profiles using LCHuman ceRNA microarray, and to verify their expression with qRT-PCR. Two-hundred-eleven DElncRNAs and one-hundred-one DEmRNAs were detected in the ceRNA microarray of ICH patients. The results of functional enrichment analysis showed that the immune response was an important part of the pathological process of ICH. Twelve lncRNAs, ten miRNAs, and seven mRNAs were present in our constructed immune-related ceRNA network, combining weighted gene co-expression network analysis (WGCNA). Our study was the first to establish the network of the immune-related ceRNAs derived from WGCNA, and to identify leukemia inhibitory factor (LIF) and B cell lymphoma 2-like 13 (BCL2L13) as pivotal immune-related biomarkers in the peripheral blood of ICH patients, which are likely associated with PI3K-Akt, the MAPK signaling pathway, and oxidative phosphorylation. The MOXD2P-miR-211-3p -LIF and LINC00299-miR-198-BCL2L13 axes were indicated to participate in the immune regulatory mechanism of ICH. The goal of our study was to offer innovative insights into the underlying immune regulatory mechanism and to identify possible immune intervention targets for ICH.
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影响因子：
5.3
作者：
Cubells JF;Sun X;Li W;Bonsall RW;McGrath JA;Avramopoulos D;Lasseter VK;Wolyniec PS;Tang YL;Mercer K;Pulver AE;Elston RC
通讯作者：
Elston RC
WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
8.2
作者：
An SJ;Kim TJ;Yoon BW
通讯作者：
Yoon BW
影响因子：
48
作者：
Li T;Wernersson R;Hansen RB;Horn H;Mercer J;Slodkowicz G;Workman CT;Rigina O;Rapacki K;Stærfeldt HH;Brunak S;Jensen TS;Lage K
通讯作者：
Lage K
影响因子：
6.1
作者：
Lu, Xi;Zhang, Hui-Yuan;He, Zhi-Yi
通讯作者：
He, Zhi-Yi