Biochemical characterization of an acidophilic β-mannanase from Gloeophyllum trabeum CBS900.73 with significant transglycosylation activity and feed digesting ability.
Biochemical characterization of an acidophilic β-mannanase from Gloeophyllum trabeum CBS900.73 with significant transglycosylation activity and feed digesting ability.
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来自 Gloeophyllum trabeum CBS900.73 的嗜酸性 β-甘露聚糖酶的生化特征，具有显着的转糖基活性和饲料消化能力。
DOI：
10.1016/j.foodchem.2015.10.115
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发表时间：
2016-04
期刊：
影响因子：
8.8
通讯作者：
罗会颖
中科院分区：
文献类型：
--
作者：
罗会颖
嗜酸性β-甘露聚糖酶由于其在极端酸性条件下的优异活性和重要的工业应用而受到广泛关注。本研究从密粘褶菌CBS900.73中克隆了糖苷水解酶家族5的β-甘露聚糖酶基因man 5A，并在毕赤酵母中成功表达。纯化的重组Man 5A是嗜酸性的，最适pH为2.5，并表现出良好的pH适应性和稳定性（分别在pH 2.0-6.0和pH 2.0-10.0范围内>80%的活性）。该酶对刺槐豆胶具有较高的比活力（1356 U/mg），能以经典的四位点结合模式降解半乳甘露聚糖和葡甘露聚糖，并能催化甘露糖转糖基化生成聚合度较高的甘露寡糖。此外，在模拟猪胃肠道条件下，它对胃蛋白酶和胰蛋白酶以及消化的玉米-豆粕基日粮具有与商业β-甘露聚糖酶相当的抗性。这种嗜酸性β-甘露聚糖酶代表了广泛应用于各种工业，特别是饲料中的有价值的候选者。
Acidophilic β-mannanases have been attracting much attention due to their excellent activity under extreme acidic conditions and significant industrial applications. In this study, a β-mannanase gene of glycoside hydrolase family 5,man5A, was cloned fromGloeophyllum trabeumCBS900.73, and successfully expressed inPichia pastoris. Purified recombinant Man5A was acidophilic with a pH optimum of 2.5 and exhibited great pH adaptability and stability (>80% activity over pH 2.0–6.0 and pH 2.0–10.0, respectively). It had a high specific activity (1356 U/mg) against locust bean gum, was able to degrade galactomannan and glucomannan in a classical four-site binding mode, and catalyzed the transglycosylation of mannotetrose to mannooligosaccharides with higher degree of polymerization. Besides, it had great resistance to pepsin and trypsin and digested corn–soybean meal based diet in a comparable way with a commercial β-mannanase under the simulated gastrointestinal conditions of pigs. This acidophilic β-mannanase represents a valuable candidate for wide use in various industries, especially in the feed.
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DOI：
--
发表时间：
2012
期刊：
Chinese Journal of Animal Nutrition
影响因子：
--
作者：
Zhang Hongfu
通讯作者：
Zhang Hongfu
影响因子：
5
作者：
Luo, Huiying;Wang, Yaru;Yao, Bin
通讯作者：
Yao, Bin
影响因子：
6.1
作者：
Junqi Zhao;P. Shi;Huiying Luo;Peilong Yang;Heng Zhao;Yingguo Bai;Huo-qing Huang;Hui Wang;B. Yao
通讯作者：
Junqi Zhao;P. Shi;Huiying Luo;Peilong Yang;Heng Zhao;Yingguo Bai;Huo-qing Huang;Hui Wang;B. Yao
影响因子：
5.7
作者：
Hilge, M;Gloor, SM;Piontek, K
通讯作者：
Piontek, K
影响因子：
5
作者：
Springer-Verlag;Berlin Heidelberg
通讯作者：
Springer-Verlag;Berlin Heidelberg