Circular RNA of cattle casein genes are highly expressed in bovine mammary gland.
Circular RNA of cattle casein genes are highly expressed in bovine mammary gland.
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牛酪蛋白基因的环状RNA在牛乳腺中高表达。
DOI：
10.3168/jds.2015-10381
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Chen Hong
中科院分区：
文献类型：
--
作者：
Zhang ChunLei;Wu Hui;Wang YanHong;Zhu ShiQi;Liu JunQiang;Fang XingTang;Chen Hong
最近的研究表明，除了激素和其他蛋白质因子外，非编码RNA分子在乳蛋白合成中发挥着重要的调节作用。环状RNA（circRNA）是普遍表达的非编码RNA种类，最近被提议调节其亲本基因的表达。在本研究中，使用称为 RNA-seq 的深度 RNA 测序技术来比较产后第 90 天和第 250 天牛乳腺的 2 个混合 RNA 样本的 circRNA 表达谱，并鉴定与泌乳有关的关键 circRNA。在产后第 90 天和第 250 天的奶牛乳腺中分别鉴定出 4,804 个和 4,048 个 circRNA，其中只有 2,231 个 circRNA 在两个泌乳阶段共表达，表明 circRNA 具有较高的阶段特异性。一些 circRNA 亲本基因的基因本体术语的富集在哺乳期之间存在差异。在前 10 个丰富的基因本体术语中，囊泡、内质网和线粒体腔在哺乳期第 90 天更为常见。所有 4 个酪蛋白编码基因（CSN1S1、CSN1S2、CSN2 和 CSN3）在牛乳腺中产生 circRNA。总共从这 4 个基因中鉴定出了 80 个 circRNA；来自CSN1S1的circRNA具有非常高的丰度，其中3个占哺乳期第90天乳腺表达的所有circRNA的36%。定量PCR证实，来自CSN1S1的3个circRNA、来自CSN1S2的1个circRNA和来自CSN2的1个circRNA在哺乳期第90天的表达量均高于哺乳期第250天的表达量。这些 circRNA 有几个 microRNA miR-2284 家族的靶位点，预计会靶向 CSN1S1 和 CSN2 mRNA，表明它们可能参与调节酪蛋白基因的表达。
Recent studies have revealed that, in addition to hormones and other protein factors, noncoding RNA molecules play an important regulatory role in milk protein synthesis. Circular RNAs (circRNAs) are universally expressed noncoding RNA species that have been proposed recently to regulate the expression of their parental genes. In the present study, the deep RNA-sequencing technique known as RNA-seq was used to compare expression profiles of circRNAs from 2 pooled RNA samples from cow mammary gland on d 90 and 250 postpartum and to identify the key circRNAs involved in lactation. A total of 4,804 and 4,048 circRNAs were identified in the cow mammary gland on d 90 and 250 postpartum, respectively, of which only 2,231 circRNAs were co-expressed at both lactation stages, suggesting high stage specificity in the circRNAs. The enrichment of some Gene Ontology terms for the circRNA parental genes differed between lactation stages. Among the top 10 enriched Gene Ontology terms, vesicle, endoplasmic reticulum, and mitochondrial lumen were more common on lactation d 90. All 4 casein-coding genes (CSN1S1, CSN1S2, CSN2, and CSN3) produced circRNAs in the cattle mammary gland. In total, 80 circRNAs were identified from these 4 genes; circRNAs from CSN1S1 had very high abundance, and 3 of them accounted for 36% of all the circRNAs expressed in the mammary gland on lactation d 90. Three circRNAs from CSN1S1, 1 circRNA from CSN1S2, and 1 circRNA from CSN2 were all more highly expressed on lactation d 90 than on lactation d 250, as confirmed by quantitative PCR. These circRNAs had several target sites for the microRNA miR-2284 family and were predicted to target CSN1S1 and CSN2 mRNA, suggesting their potential involvement in regulating expression of the casein genes.
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影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
DOI：
10.1073/pnas.1210057109
发表时间：
2012-10-16
影响因子：
11.1
作者：
Jabed, Anower;Wagner, Stefan;Laible, Goetz
通讯作者：
Laible, Goetz
影响因子：
--
作者：
Li F;Zhang L;Li W;Deng J;Zheng J;An M;Lu J;Zhou Y
通讯作者：
Zhou Y
影响因子：
4.2
作者：
Schaafsma, G
通讯作者：
Schaafsma, G
DOI：
10.1007/978-1-4614-4714-6_16
发表时间：
2013
期刊：
--
影响因子：
--
作者：
L. Pellegrino;F. Masotti;S. Cattaneo;J. Hogenboom;I. D. Noni
通讯作者：
L. Pellegrino;F. Masotti;S. Cattaneo;J. Hogenboom;I. D. Noni