Circular RNA ITCH has inhibitory effect on ESCC by suppressing the Wnt/β-catenin pathway.
Circular RNA ITCH has inhibitory effect on ESCC by suppressing the Wnt/β-catenin pathway.
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DOI：
10.18632/oncotarget.3469
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发表时间：
2015-03-20
期刊：
影响因子：
--
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Li F;Zhang L;Li W;Deng J;Zheng J;An M;Lu J;Zhou Y
具有外显子序列的环状rna是一种特殊形式的非编码rna，是通过分析少数转录基因发现的。已经观察到环状rna具有微rna海绵的功能。在本研究中，我们研究了环状rna的表达是否在食管鳞状细胞癌（ESCC）的发展过程中发生改变。采用基于taqman的逆转录酶聚合酶链反应方法，分析了来自中国东部和南部的684例ESCC和配对的非肿瘤组织样本中cirr - itch与ESCC之间的关系。我们发现，与肿瘤周围组织相比，ciri - itch在ESCC中的表达通常较低。通过生化分析进一步检测cirr - itch的功能相关性。circ -ITCH作为miR-7、miR-17和miR-214的海绵，可能会增加ITCH的水平。ITCH高表达促进泛素化和磷酸化Dvl2的降解，从而抑制Wnt/β-catenin通路。这些结果表明cirr - itch可能通过调节Wnt通路对ESCC有抑制作用。
Circular RNAs with exonic sequences represent a special form of non-coding RNAs, discovered by analyzing a handful of transcribed genes. It has been observed that circular RNAs function as microRNA sponges. In the present study, we investigated whether the expression of circular RNAs is altered during the development of esophageal squamous cell carcinoma (ESCC). Using a TaqMan-based reverse transcriptase polymerase chain reaction assay, the relationship between cir-ITCH and ESCC was analyzed in a total of 684 ESCC and paired adjacent non-tumor tissue samples from eastern and southern China. We found that cir-ITCH expression was usually low in ESCC compared to the peritumoral tissue. The functional relevance of cir-ITCH was further examined by biochemical assays. As sponge of miR-7, miR-17, and miR-214, cir-ITCH might increase the level of ITCH. ITCH hyper expression promotes ubiquitination and degradation of phosphorylated Dvl2, thereby inhibiting the Wnt/β-catenin pathway. These results indicate that cir-ITCH may have an inhibitory effect on ESCC by regulating the Wnt pathway.
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影响因子：
14.9
作者：
Danan M;Schwartz S;Edelheit S;Sorek R
通讯作者：
Sorek R
miR-216b通过靶向KRAS抑制鼻咽癌中的肿瘤生长和侵袭
影响因子：
4
作者：
Deng, Min;Tang, Hailin;Li, Guiyuan
通讯作者：
Li, Guiyuan
DOI：
10.1073/pnas.060025397
发表时间：
2000-04-11
影响因子：
11.1
作者：
Lin, SY;Xia, WY;Hung, MC
通讯作者：
Hung, MC
影响因子：
11.2
作者：
Chou, Yu-Ting;Lin, Hua-Heng;Wu, Cheng-Wen
通讯作者：
Wu, Cheng-Wen
影响因子：
30.8
作者：
Perry, WL;Hustad, CM;Copeland, NG
通讯作者：
Copeland, NG