Characterization of total membrane protein of Porphyromonas endodontalis.
Characterization of total membrane protein of Porphyromonas endodontalis.
复制标题
牙髓卟啉单胞菌总膜蛋白的表征。
DOI：
10.1016/s0099-2399(06)81334-8
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发表时间：
1992
影响因子：
4.2
通讯作者：
Neiders,ME
中科院分区：
文献类型：
--
作者：
Herweijer,JA;Loos,BG;Neiders,ME
将牙髓卟啉单胞菌菌株ATCC 35406、HG 181和HG 413在富集肉汤培养基中培养，并测定生长曲线。用十二烷基硫酸钠聚丙烯酰胺凝胶电泳研究了从指数期或生长稳定期收获的细胞总膜蛋白谱。蛋白质谱表明，这三个菌株共有三个主要蛋白质，命名为A，B和C，分子量分别为59，43和41 kDa。此外，菌株中的大多数次要蛋白质具有同源性。然而，每个菌株的一些次要蛋白质是独特的。两种常见的次要蛋白质的分子量为56和51 kDa的弱表达或不存在于膜制剂从细胞的早期指数期，而它们存在于膜制剂从细胞收获的生长后期。主膜蛋白和次膜蛋白可能在P.牙髓和宿主。此外，膜蛋白可能在牙髓病病原菌的生理过程中发挥重要作用。
Porphyromonas endodontalisstrains ATCC 35406, HG 181, and HG 413 were cultured in an enriched broth medium, and growth curves were determined. Total membrane protein profiles of cells, harvested either from exponential phase or from stationary phase of growth, were studied by sodium dodecyl sulfate polyacrylamide-gel electrophoresis. The protein profiles indicated that the three strains shared three major proteins, designated A, B, and C, with molecular masses of 59, 43, and 41 kDa, respectively. In addition, the strains showed homology among the majority of the minor proteins. However, some minor proteins were unique for each strain. Two common minor proteins with molecular masses of 56 and 51 kDa were weakly expressed or absent in membrane preparations from cells of the early exponential phase, while they were present in membrane preparations from cells harvested in the late phase of growth. The major and minor membrane proteins may play a role in the interaction betweenP. endodontalisand the host. In addition, the membrane proteins may play an important role in the physiology of this endodontic pathogen.
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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作者：
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