Determination and characterization of bovine interleukin-17 cDNA.
Determination and characterization of bovine interleukin-17 cDNA.
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牛白细胞介素 17 cDNA 的测定和表征。
DOI：
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发表时间：
2006
影响因子：
2.3
通讯作者：
P. Rainard
中科院分区：
文献类型：
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作者：
C. Riollet;D. Mutuel;M. Duonor;P. Rainard
白细胞介素-17（IL-17）是由激活的记忆T细胞产生的促炎细胞因子，并且似乎通过刺激基质细胞分泌其它细胞因子而在T细胞触发的炎症中发挥上游作用。我们假设IL-17在感染或免疫介导的炎症过程中在牛乳腺中性粒细胞的募集中起作用。利用cDNA末端快速扩增（RACE）方法获得牛IL-17（BoIL-17）cDNA，该cDNA含有一个462 bp的开放阅读框架（ORF），编码153个氨基酸（aa），分子量为17.2 kDa，一个23个氨基酸的NH（2）末端信号肽，一个潜在的N-糖基化位点和6个半胱氨酸残基。BoIL-17蛋白与人蛋白的同源性为73.5%，与小鼠和大鼠蛋白的同源性为67%。用BoIL-17 cDNA转染Sf 9昆虫细胞，并在牛乳腺上皮细胞（MEC）的原代培养物上测试上清液的生物活性。诱导IL-6、IL-8和生长相关癌基因α（Groalpha）的mRNA合成，表明IL-17在乳腺免疫中的功能作用。
Interleukin-17 (IL-17) is a proinflammatory cytokine produced by activated memory T cells, and it appears to play an upstream role in T cell-triggered inflammation by stimulating stromal cells to secrete other cytokines. We hypothesize that IL-17 plays a role in the recruitment of neutrophils in the bovine mammary gland during infection or immune-mediated inflammation. The rapid amplification of cDNA ends (RACE) method was used to obtain a cDNA of bovine IL-17 (BoIL-17) containing a 462-bp open reading frame (ORF) encoding a protein of 153 amino acids (aa) with a molecular mass of 17.2 kDa, a 23-residue NH(2)-terminal signal peptide, a single potential N-linked glycosylation site, and 6 cysteine residues. BoIL-17 protein shared 73.5% identity with the human protein and 67% with the mouse and rat proteins. Sf9 insect cells were transfected with BoIL-17 cDNA, and supernatant was tested for biologic activity on a primary culture of bovine mammary epithelial cells (MECs). mRNA synthesis of IL-6, IL-8, and growth-related oncogene alpha (Groalpha) was induced, suggesting a functional role for IL-17 in mammary immunity.
DOI：
10.1165/ajrcmb.25.3.4424
发表时间：
2001-09-01
影响因子：
6.4
作者：
Ye, P;Garvey, PB;Kolls, JK
通讯作者：
Kolls, JK
囊性纤维化患者外周血单核细胞弹性蛋白酶分泌增加。
影响因子：
4.6
作者：
Jones,MM;Seilheimer,DK;Pier,GB;Rossen,RD
通讯作者：
Rossen,RD
DOI：
10.1073/pnas.83.6.1670
发表时间：
1986-03-01
影响因子：
11.1
作者：
CAPUT, D;BEUTLER, B;CERAMI, A
通讯作者：
CERAMI, A