Transcriptome profiling of male and female Ascaris lumbricoides reproductive tissues.
Transcriptome profiling of male and female Ascaris lumbricoides reproductive tissues.
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DOI：
10.1186/s13071-022-05602-2
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发表时间：
2022-12-20
影响因子：
3.2
通讯作者：
Adisakwattana, Poom
中科院分区：
文献类型：
--
作者：
Phuphisut, Orawan;Poodeepiyasawat, Akkarin;Yoonuan, Tippayarat;Watthanakulpanich, Dorn;Chotsiri, Palang;Reamtong, Onrapak;Mousley, Angela;Gobert, Geoffrey N. N.;Adisakwattana, Poom
类蚓蛔虫引起人类蛔虫病，这是最普遍的寄生虫病，全球约有10亿人感染。2019年，全球疾病负担估计为75.4万残疾调整生命年，导致2090人死亡。在缺乏疫苗接种策略的情况下，蛔虫病的治疗依赖于驱虫化疗，但耐药性是一个问题。再感染的倾向也是对疾病控制的一个主要挑战；雌虫每天产下多达20万个卵，这些卵会污染周围环境，并存活数年，导致高传播率。了解生殖过程的分子机制，包括控制卵子产生、精子发生、卵子发生和胚胎发生，将推动未来控制蛔虫病的新药和/或疫苗靶点的开发。利用Illumina HiSeq软件，通过2 × 150 bp对端测序，获得了成虫雄性和雌性的离散生殖和体细胞组织样本的转录组图谱。男性组织包括：睾丸生发带、睾丸输精管部分、精囊和躯体组织。雌性组织包括：卵巢生发带、卵巢部分输卵管、子宫和体细胞组织。差异表达基因（DEGs）从每千碱基每百万读取片段（FPKM）图谱中鉴定出来。进行分层分析以鉴定组织特异性基因。此外，采用基因本体（GO）和京都基因与基因组百科全书（KEGG）分析来识别deg的重要术语和途径。研究表明，参与蛋白磷酸化和粘附分子的deg分别在精子发生和受精中起着至关重要的作用。这些与g蛋白偶联受体（GPCR）信号通路和gtpase介导的小信号转导通路相关的基因在卵发生过程中对细胞骨架组织起重要作用。此外，与SMA基因和TGF-β信号通路相关的deg在成年女性胚胎发生中起着至关重要的作用。在本研究中发现的与特定生物学过程和途径相关的一些基因与种系发育、胚胎发生和生殖行为的缺陷有关。在富集的KEGG通路分析中，发现Hippo信号通路、催产素信号通路和紧密连接通路在蛔虫雌雄生殖系统中发挥作用。本研究提供了离散的蚓类生殖组织样本的全面转录组图谱，揭示了这些功能重要组织的分子基础。这项研究产生的数据将提供蛔虫生殖生物学的基础知识，并将为未来确定抗蛔虫药物和/或疫苗的靶点提供信息。在线版本包含补充材料，可在10.1186/ s130771 -022-05602-2获得。
Ascaris lumbricoides causes human ascariasis, the most prevalent helminth disease, infecting approximately 1 billion individuals globally. In 2019 the global disease burden was estimated to be 754,000 DALYs and resulted in 2090 deaths. In the absence of a vaccination strategy, treatment of ascariasis has relied on anthelminthic chemotherapy, but drug resistance is a concern. The propensity for reinfection is also a major challenge to disease control; female worms lay up to 200,000 eggs daily, which contaminate surrounding environments and remain viable for years, resulting in high transmission rates. Understanding the molecular mechanisms of reproductive processes, including control of egg production, spermatogenesis, oogenesis and embryogenesis, will drive the development of new drugs and/or vaccine targets for future ascariasis control. Transcriptome profiles of discrete reproductive and somatic tissue samples were generated from adult male and female worms using Illumina HiSeq with 2 × 150 bp paired-end sequencing. Male tissues included: testis germinal zone, testis part of vas deferens, seminal vesicle and somatic tissue. Female tissues included: ovary germinal zone, ovary part of the oviduct, uterus and somatic tissue. Differentially expressed genes (DEGs) were identified from the fragments per kilobases per million reads (FPKM) profiles. Hierarchical analysis was performed to identify tissue-specific genes. Furthermore, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were employed to identify significant terms and pathways for the DEGs. DEGs involved in protein phosphorylation and adhesion molecules were indicated to play a crucial role in spermatogenesis and fertilization, respectively. Those genes associated with the G-protein-coupled receptor (GPCR) signaling pathway and small GTPase-mediated signal transduction pathway play an essential role in cytoskeleton organization during oogenesis. Additionally, DEGs associated with the SMA genes and TGF-β signaling pathway are crucial in adult female embryogenesis. Some genes associated with particular biological processes and pathways that were identified in this study have been linked to defects in germline development, embryogenesis and reproductive behavior. In the enriched KEGG pathway analysis, Hippo signaling, oxytocin signaling and tight junction pathways were identified to play a role in Ascaris male and female reproductive systems. This study has provided comprehensive transcriptome profiles of discrete A. lumbricoides reproductive tissue samples, revealing the molecular basis of these functionally important tissues. The data generated from this study will provide fundamental knowledge on the reproductive biology of Ascaris and will inform future target identification for anti-ascariasis drugs and/or vaccines. The online version contains supplementary material available at 10.1186/s13071-022-05602-2.
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DOI：
10.1590/s0001-37652011000200022
发表时间：
2011-06-01
期刊：
Anais da Academia Brasileira de Ciências
影响因子：
--
作者：
Dissous, Colette;Grevelding, Christoph G;Long, Thavy
通讯作者：
Long, Thavy
影响因子：
6.7
作者：
Beckmann S;Buro C;Dissous C;Hirzmann J;Grevelding CG
通讯作者：
Grevelding CG
CYK-4：中央纺锤体形成和细胞因子所需的RHO家族GTPase激活蛋白（GAP）。
影响因子：
7.8
作者：
Jantsch-Plunger, V;Gonczy, P;Romano, A;Schnabel, H;Hamill, D;Schnabel, R;Hyman, A A;Glotzer, M
通讯作者：
Glotzer, M
DOI：
10.1126/science.1226201
发表时间：
2012-10-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Garrison JL;Macosko EZ;Bernstein S;Pokala N;Albrecht DR;Bargmann CI
通讯作者：
Bargmann CI
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y