RNA-Seq analysis reveals that multiple phytohormone biosynthesis and signal transduction pathways are reprogrammed in curled-cotyledons mutant of soybean [Glycine max (L.) Merr].
RNA-Seq analysis reveals that multiple phytohormone biosynthesis and signal transduction pathways are reprogrammed in curled-cotyledons mutant of soybean [Glycine max (L.) Merr].
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RNA-Seq 分析揭示，大豆卷曲子叶突变体 [Glycine max (L.) Merr.] 中多种植物激素生物合成和信号转导途径被重新编程
DOI：
10.1186/1471-2164-15-510
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发表时间：
2014-06-21
期刊：
影响因子：
4.4
通讯作者：
Yu D
中科院分区：
文献类型：
--
作者：
Shi G;Huang F;Gong Y;Xu G;Yu J;Hu Z;Cai Q;Yu D
大豆是世界上最重要的经济作物之一。子叶是种子中营养物质的储存区，对种子质量和产量至关重要。子叶突变体对于胚胎形成和种子发育的遗传解剖是重要的。然而，大豆子叶发育的分子机制在很大程度上是unexplored.ResultsIn这项研究中，我们的特点是soybeancurled-cotyledon（CCO）突变体。与野生型（WT）相比，鱼雷期的子叶变得更细长，并向外生长。突变体的整个胚胎类似于“燕尾”。此外，种子发芽率和赤霉酸（GA 3）水平降低，而脱落酸（阿坝）和生长素（IAA）水平增加。RNA-seq在WT和对照之间鉴定了1，093个差异表达基因（DEG）。KEGG途径分析表明，DEG参与了激素合成和信号转导途径。与种子中激素的测定一致，RNA-seq的结果表明，生长素和阿坝的生物合成和信号传导比WT更活跃，而GA生物合成的早期步骤被阻断，以及GA信号传导中的非活性GA向活性GA的转化率。此外，参与细胞分裂素（CK）、乙烯（ET）、油菜素内酯（BR）和茉莉酸（JA）等激素生物合成和信号转导途径的基因也受到影响。表明大豆子叶发育过程中存在多种激素的协同作用。
BackgroundSoybean is one of the most economically important crops in the world. The cotyledon is the nutrient storage area in seeds, and it is critical for seed quality and yield. Cotyledon mutants are important for the genetic dissection of embryo patterning and seed development. However, the molecular mechanisms underlying soybean cotyledon development are largely unexplored.ResultsIn this study, we characterised a soybeancurled-cotyledon(cco) mutant. Compared with wild-type (WT), anatomical analysis revealed that theccocotyledons at the torpedo stage became more slender and grew outward. The entire embryos ofccomutant resembled the “tail of swallow”. In addition,ccoseeds displayed reduced germination rate and gibberellic acid (GA3) level, whereas the abscisic acid (ABA) and auxin (IAA) levels were increased. RNA-seq identified 1,093 differentially expressed genes (DEGs) between WT and theccomutant. The KEGG pathway analysis showed many DEGs were mapped to the hormone biosynthesis and signal transduction pathways. Consistent with assays of hormones in seeds, the results of RNA-seq indicated auxin and ABA biosynthesis and signal transduction inccowere more active than in WT, while an early step in GA biosynthesis was blocked, as well as conversion rate of inactive GAs to bioactive GAs in GA signaling. Furthermore, genes participated in other hormone biosynthesis and signalling pathways such as cytokinin (CK), ethylene (ET), brassinosteroid (BR), and jasmonate acid (JA) were also affected in theccomutant.ConclusionsOur data suggest that multiple phytohormone biosynthesis and signal transduction pathways are reprogrammed incco, and changes in these pathways may partially contribute to theccomutant phenotype, suggesting the involvement of multiple hormones in the coordination of soybean cotyledon development.
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影响因子：
5.1
作者：
Brugiere, Norbert;Humbert, Sabrina;Habben, Jeffrey E.
通讯作者：
Habben, Jeffrey E.
影响因子：
7.2
作者：
CHAUDHURY, AM;LETHAM, S;DENNIS, ES
通讯作者：
DENNIS, ES
影响因子：
10.5
作者：
Cheng, Youfa;Dai, Xinhua;Zhao, Yunde
通讯作者：
Zhao, Yunde
影响因子：
11.6
作者：
Banno, H;Ikeda, Y;Chua, NH
通讯作者：
Chua, NH
影响因子：
4.6
作者：
Furutani, M;Vernoux, T;Aida, M
通讯作者：
Aida, M