Kallikrein-related peptidase 4, matrix metalloproteinase 20, and the maturation of murine and porcine enamel.
Kallikrein-related peptidase 4, matrix metalloproteinase 20, and the maturation of murine and porcine enamel.
复制标题
DOI：
10.1111/j.1600-0722.2011.00859.x
复制
发表时间：
2011-12
影响因子：
1.9
通讯作者：
Simmer JP
中科院分区：
文献类型：
--
作者：
Hu Y;Hu JC;Smith CE;Bartlett JD;Simmer JP
基质金属蛋白酶20（Mmp 20）缺失小鼠的牙冠在牙本质-釉质连接（DEJ）处断裂，而激肽释放酶相关肽酶4（Klk 4）缺失小鼠的牙冠在DEJ正上方的深层釉质中断裂。我们使用背散射扫描电子显微镜来评估9周龄野生型、Klk 4缺失和Mmp 20缺失小鼠的切牙以及发育中的猪磨牙中的釉质矿化。我们观察到一条沿着DEJ的线在发育中的野生型小鼠和猪的牙齿矿化过度。这条线是可辨别的，从早期分泌阶段，直到在成熟阶段的釉质达到类似的密度。该线在Klk 4缺失小鼠中是明显的，但在Mmp 20缺失小鼠中不存在。Klk 4缺失小鼠的牙釉质在表面正常成熟，但随着深度的增加，矿化程度逐渐降低。在Mmp 20敲除小鼠中的釉质形成为矿物质双层，没有一层看起来像真正的釉质。最表层的矿物层在成熟阶段膨胀，形成不规则的表面结核。一个令人惊讶的发现是观察到的电子背散射从成熟中期的野生型成釉细胞，我们归因于铁的积累和释放。我们的结论是，由于随着深度的增加釉质成熟度降低，Klk 4基因缺失小鼠的深层釉质中的釉质断裂，而Mmp 20基因缺失小鼠的DEJ处的釉质断裂是由于DEJ处的矿化不足。
The crowns of matrix metalloproteinase 20 (Mmp20) null mice fracture at the dentino–enamel junction (DEJ), whereas the crowns of kallikrein-related peptidase 4 (Klk4) null mice fracture in the deep enamel just above the DEJ. We used backscatter scanning electron microscopy to assess enamel mineralization in incisors from 9-wk-old wild-type, Klk4 null, and Mmp20 null mice, and in developing pig molars. We observed a line of hypermineralization along the DEJ in developing wild-type mouse and pig teeth. This line was discernible from the early secretory stage until the enamel in the maturation stage reached a similar density. The line was apparent in Klk4 null mice, but absent in Mmp20 null mice. Enamel in the Klk4 null mice matured normally at the surface, but was progressively less mineralized with depth. Enamel in the Mmp20 null mice formed as a mineral bilayer, with neither layer looking like true enamel. The most superficial mineral layer expanded during the maturation stage and formed irregular surface nodules. A surprising finding was the observation of electron backscatter from mid-maturation wild-type ameloblasts, which we attributed to the accumulation and release of iron. We conclude that enamel breaks in the deep enamel of Klk4 null mice because of decreasing enamel maturation with depth, and at the DEJ in Mmp20 null mice because of hypomineralization at the DEJ.
登录
查看更多内容
影响因子：
3
作者：
Beniash, Elia;Metzler, Rebecca A.;Lam, Raymond S. K.;Gilbert, P. U. P. A.
通讯作者：
Gilbert, P. U. P. A.
影响因子：
4.8
作者：
Simmer, James P.;Hu, Yuanyuan;Hu, Jan C. -C.
通讯作者：
Hu, Jan C. -C.
影响因子：
7.6
作者：
Chun YH;Yamakoshi Y;Yamakoshi F;Fukae M;Hu JC;Bartlett JD;Simmer JP
通讯作者：
Simmer JP
影响因子：
4.8
作者：
Hu, Jan C. -C.;Hu, Yuanyuan;Simmer, James P.
通讯作者：
Simmer, James P.
影响因子：
4.8
作者：
Caterina, JJ;Skobe, Z;Bartlett, JD
通讯作者：
Bartlett, JD