Proteolytic enhancement of human rotavirus infectivity.
Proteolytic enhancement of human rotavirus infectivity.
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人轮状病毒感染性的蛋白水解增强。
DOI：
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发表时间：
1993
影响因子：
11.8
通讯作者：
Y. Numazaki
中科院分区：
文献类型：
--
作者：
T. Konno;H. Suzuki;S. Kitaoka;T. Sato;N. Fukuhara;O. Yoshie;K. Fukudome;Y. Numazaki
轮状病毒是世界范围内引起严重腹泻的最重要病原体。尽管疫苗开发取得了很大进展，但对免疫以外的宿主保护机制知之甚少。本报告的重点是轮状病毒感染的蛋白水解增强，重点是VP4，外壳蛋白的功能。胰蛋白酶可选择性切割VP4，从而增强人轮状病毒的体外生长。用胰蛋白酶处理增加了人轮状病毒的感染性，同时降低了其血凝活性。轮状病毒内化有两种模式：在胰蛋白酶的帮助下直接穿透和在没有胰蛋白酶的情况下内吞。通过被胰蛋白酶切割的VP4的直接穿透对于病毒的复制是必不可少的，而内吞内化不引起病毒复制。
Rotaviruses are the most important etiologic agents of severe diarrhea worldwide. Despite great advances in vaccine development, little is known about host protection mechanisms other than immunity. This presentation focuses on the proteolytic enhancement of rotavirus infection, with emphasis on the functions of VP4, an outer capsid protein. The in vitro growth of human rotavirus is enhanced by trypsin, which selectively cleaves VP4. Treatment with trypsin increases the infectivity of human rotavirus while decreasing its hemagglutination activity. There are two modes of rotavirus internalization: direct penetration with the aid of trypsin and endocytosis without trypsin. Direct penetration via VP4 cleaved by trypsin is essential for the replication of the virus, whereas endocytotic internalization does not give rise to viral replication.
影响因子：
5.6
作者：
PRASAD, BVV;WANG, GJ;CHIU, W
通讯作者：
CHIU, W