Functional analysis of the Nep1-like proteins from Plasmopara viticola.
Functional analysis of the Nep1-like proteins from Plasmopara viticola.
复制标题
葡萄生单轴霉 Nep1 样蛋白的功能分析
DOI：
10.1080/15592324.2021.2000791
复制
发表时间：
2022-12-31
影响因子：
2.9
通讯作者：
Wu, Jiang
中科院分区：
文献类型：
--
作者：
Xiang, Jiang;Cheng, Jianhui;Wei, Lingzhu;Li, Mingshan;Wu, Jiang
坏死和乙烯诱导肽1 （Nep1）样蛋白（NLP）是由多种分类无关的植物病原体（细菌、真菌和卵菌）分泌的，在双子叶植物中以诱导细胞死亡和免疫反应而闻名。我们在前期研究中用RNA-Seq预测了一组来自专性生物营养性卵菌Plasmopara viticola的推定NLP基因，但其活性尚未得到证实。因此，我们分析了葡萄假单胞菌NLP （PvNLP）家族，鉴定出7个PvNLP基因。与其他病原菌NLP基因相比，它们均属于1型NLP基因，形成葡萄假单胞菌特异性集群。pvnlp在感染早期即被诱导表达，其表达模式可分为两组。农杆菌介导的瞬时表达实验显示，只有PvNLP7具有细胞毒性，并能诱导本菌烟草对辣椒疫霉产生抗性。功能分析显示，PvNLP4、PvNLP5、PvNLP7和PvNLP10显著提高拟南芥对拟南芥透明膜孢子虫的抗病性。此外，当这四个基因在拟南芥中过度表达时，会导致植物生长抑制，这通常与免疫增强有关。进一步研究发现，PvNLP7可以激活防御相关基因的表达，其保守的NPP1结构域对细胞死亡和免疫诱导活性至关重要。这一记录显示了葡萄霉NLP基因的功能多样化，为进一步研究葡萄霉的致病机制奠定了基础。
ABSTRACT Necrosis and ethylene-inducing peptide 1 (Nep1) -like proteins (NLP) are secreted by multiple taxonomically unrelated plant pathogens (bacteria, fungi, and oomycete) and are best known for inducing cell death and immune responses in dicotyledonous plants. A group of putative NLP genes from obligate biotrophic oomycete Plasmopara viticola were predicted by RNA-Seq in our previous study, but their activity has not been established. Therefore, we analyzed the P. viticola NLP (PvNLP) family and identified seven PvNLP genes. They all belong to type 1 NLP genes and form a P. viticola-specific cluster when compared with other pathogen NLP genes. The expression of PvNLPs was induced during early infection process and the expression patterns could be categorized into two groups. Agrobacterium tumefaciens-mediated transient expression assays revealed that only PvNLP7 was cytotoxic and could induce Phytophthora capsici resistance in Nicotiana benthamiana. Functional analysis showed that PvNLP4, PvNLP5, PvNLP7, and PvNLP10 significantly improved disease resistance of Arabidopsis thaliana to Hyaloperonospora arabidopsidis. Moreover, the four genes caused an inhibition of plant growth which is typically associated with enhanced immunity when over-expressed in Arabidopsis. Further research found that PvNLP7 could activate the expression of defense-related genes and its conserved NPP1 domain was critical for cell death- and immunity-inducing activity. This record of NLP genes from P. viticola showed a functional diversification, laying a foundation for further study on pathogenic mechanism of the devastating pathogen.
登录
查看更多内容
影响因子：
7.4
作者：
Bae, Hanhong;Kim, Moon S.;Bailey, Bryan A.
通讯作者：
Bailey, Bryan A.
影响因子：
46.9
作者：
Amsellem, Z;Cohen, BA;Gressel, J
通讯作者：
Gressel, J
影响因子：
7.2
作者：
Fellbrich, G;Romanski, A;Nürnberger, T
通讯作者：
Nürnberger, T
影响因子：
3.5
作者：
Kanneganti, Thirumala-Devi;Huitema, Edgar;Kamoun, Sophien
通讯作者：
Kamoun, Sophien
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF