Myocardial metabolism of 123I-BMIPP in a canine model with ischemia: implications of perfusion-metabolism mismatch on SPECT images in patients with ischemic heart disease.
Myocardial metabolism of 123I-BMIPP in a canine model with ischemia: implications of perfusion-metabolism mismatch on SPECT images in patients with ischemic heart disease.
复制标题
犬缺血模型中 123I-BMIPP 的心肌代谢：灌注代谢不匹配对缺血性心脏病患者 SPECT 图像的影响。
DOI：
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发表时间：
1999
影响因子：
9.3
通讯作者：
S. Sasayama
中科院分区：
文献类型：
--
作者：
R. Hosokawa;R. Nohara;Y. Fujibayashi;K. Okuda;M. Ogino;T. Hirai;M. Fujita;N. Tamaki;J. Konishi;S. Sasayama
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123I-(ρ-碘苯基)-3-R,S-甲基十五烷酸 (BMIPP) 是一种用于 SPECT 成像的脂肪酸类似物。这种放射性药物具有独特的性质，即缺血性心脏病患者 SPECT 图像上的灌注-代谢不匹配。然而，这种机制的原因仍不清楚。
方法
使用麻醉下的开胸狗，我们制作了一个系统，可以在必要时将心大静脉的所有血液释放到外部，而无需再循环。再灌注后将左前降支动脉(LAD)闭塞30分钟。将BMIPP注射到LAD后，从心静脉和腹主动脉（6只狗）采集血液样本或从LAD区域（5只狗）进行连续活检标本，然后与正常对照进行比较。在外排研究中，使用高效液相色谱 (HPLC) 评估了 BMIPP 的分解代谢物，包括非代谢 BMIPP 的反向扩散。薄层色谱（TLC）技术被引入组织分析研究中。
结果
尽管 BMIPP 从血浆中快速提取到心肌中以及随后的保留没有变化，但早期放射性清除（8 分钟）随着缺血而显着增加（51% +/- 12% 至 65% +/- 7%；P < 0.05）。来自心肌的代谢物由非代谢BMIPP、α、中间和完全氧化代谢物的反向扩散组成。在这些代谢物中，血液中非代谢BMIPP的反向扩散显着增加（27.9%+/-7.7%至42.3%+/-8.1%；P<0.05），特别是在缺血的早期阶段。在组织中，即使在早期，放射性也集中在甘油三酯池中，此外，BMIPP和α氧化代谢物在缺血早期显着减少（注射BMIPP后t=1分钟，25.9%+/-8.6%至14.5%+/-2.1%，P<0.01；t=2分钟，8.9%+/-5.0%至4.5%+/- 1.7%，P < 0.05）。
结论
这些结果表明，随着缺血，来自心肌的非代谢BMIPP的反向扩散增加，组织中的BMIPP（长链脂肪酸）减少，这表明非代谢BMIPP的反向扩散可能在缺血性心脏病患者SPECT图像上的心肌灌注-代谢不匹配中发挥重要作用。
UNLABELLED
123I-(rho-iodophenyl)-3-R,S-methylpentadecanoic acid (BMIPP) is a fatty acid analog for SPECT imaging. This radiopharmaceutical possesses the unique property, that is, perfusion-metabolism mismatch on SPECT images in patients with ischemic heart disease. However, the reason of this mechanism remains unclear.
METHODS
Using open-chest dogs under anesthesia, we made a system to release all the blood of the great cardiac vein outside without recirculation, if necessary. Left anterior descending artery (LAD) was occluded for 30 min after reperfusion. After the injection of BMIPP into LAD, blood samplings from the cardiac vein and abdominal aorta (6 dogs) or serial biopsy specimens from the LAD region (5 dogs) were performed, and then compared with the normal control. The catabolites of BMIPP, including backdiffusion of nonmetabolized BMIPP, were evaluated with high-performance liquid chromatography (HPLC) in the efflux study. Thin-layer chromatography (TLC) technique was introduced in the tissue analytical study.
RESULTS
Although the rapid extraction of BMIPP from the plasma into the myocardium and the subsequent retention were unchanged, the early washout (8 min) of radioactivity significantly increased (51% +/- 12% to 65% +/- 7%; P < 0.05) with ischemia. The metabolites from the myocardium consisted of backdiffusion of nonmetabolized BMIPP, alpha, intermediate, and full oxidation metabolites. Among these metabolites, backdiffusion of nonmetabolized BMIPP in blood significantly increased (27.9% +/- 7.7% to 42.3% +/- 8.1%; P < 0.05), especially in the early phase with ischemia. In tissue, the radioactivity was concentrated in the triglyceride pool even in the early phase, and in addition, BMIPP and alpha-oxidized metabolite significantly decreased in the early phase with ischemia (t = 1 min after BMIPP injection, 25.9% +/- 8.6% to 14.5% +/- 2.1%, P < 0.01; t = 2 min, 8.9% +/- 5.0% to 4.5% +/- 1.7%, P < 0.05).
CONCLUSION
These results show that backdiffusion of nonmetabolized BMIPP from the myocardium increased and BMIPP (long-chain fatty acids) in tissue decreased with ischemia, suggesting backdiffusion of nonmetabolized BMIPP might play an important role in myocardial perfusion-metabolism mismatch on SPECT images in patients with ischemic heart disease.
使用单个心外膜传感器对心肌增厚进行多普勒测量。
DOI：
10.1152/ajpheart.1983.245.6.h1066
发表时间：
1983
期刊：
The American journal of physiology
影响因子：
--
作者：
Hartley,CJ;Latson,LA;Michael,LH;Seidel,CL;Lewis,RM;Entman,ML
通讯作者：
Entman,ML