MoSfl1 is important for virulence and heat tolerance in Magnaporthe oryzae.
MoSfl1 is important for virulence and heat tolerance in Magnaporthe oryzae.
复制标题
DOI：
10.1371/journal.pone.0019951
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Xu JR
中科院分区：
文献类型：
--
作者：
Li G;Zhou X;Kong L;Wang Y;Zhang H;Zhu H;Mitchell TK;Dean RA;Xu JR
附着体的形成受MST11-MST7-PMK1 MAPK级联信号通路的调控。其下游转录因子MST12对穿透和侵袭性生长很重要，但对附着胞的形成是必不可少的。为了确定受Pmk1调控的其他下游靶点，在本研究中，我们使用由573个米分枝杆菌转录因子(TF)基因组成的蛋白质微阵列进行了磷酸化分析。用免疫共沉淀法进一步分析了Pmk1体外磷酸化的3个TF基因。其中一种是MoSFL1，在体内发现与Pmk1相互作用。与其他SFL1同源物一样，MoSfl1蛋白具有HSF样结构域。当在酵母中表达时，MoSFL1在功能上补充了SFL1突变体的絮凝缺陷。在米曲霉中，MoSFl1基因的缺失导致水稻和大麦幼苗的致病力显著降低。与此一致的是，在水稻叶鞘渗透试验中，Mosfl1突变体在侵入生长方面存在缺陷。与营养菌丝相比，附着体和感病水稻叶片中MoSFL1的表达水平均升高。Mosfl1突变体对高温的敏感度也增加了。在30℃下生长的Mosfl1和pmk1突变株的CM培养中，气生菌丝的产生和黑化减少，但其生长速度没有改变。经qRT-PCR检测，MoHSP30和MoHSP98基因在Mosfl1突变体中的转录水平分别降低10倍和3倍。SFL1同源基因在丝状子囊菌中保守，但在非酵母菌中尚未发现其功能特征。MoSfl1有一个可能的MAPK对接位点和三个可能的MAPK磷酸化位点。因此，它可能与Pmk1在调节米曲霉侵袭生长和胁迫反应中的功能有关。
The formation of appressoria, specialized plant penetration structures of Magnaporthe oryzae, is regulated by the MST11-MST7-PMK1 MAP kinase cascade. One of its downstream transcription factor, MST12, is important for penetration and invasive growth but dispensable for appressorium formation. To identify additional downstream targets that are regulated by Pmk1, in this study we performed phosphorylation assays with a protein microarray composed of 573 M. oryzae transcription factor (TF) genes. Three of the TF genes phosphorylated by Pmk1 in vitro were further analyzed by coimmunoprecipitation assays. One of them, MoSFL1, was found to interact with Pmk1 in vivo. Like other Sfl1 orthologs, the MoSfl1 protein has the HSF-like domain. When expressed in yeast, MoSFL1 functionally complemented the flocculation defects of the sfl1 mutant. In M. oryzae, deletion of MoSFl1 resulted in a significant reduction in virulence on rice and barley seedlings. Consistent with this observation, the Mosfl1 mutant was defective in invasive growth in penetration assays with rice leaf sheaths. In comparison with that of vegetative hyphae, the expression level of MoSFL1 was increased in appressoria and infected rice leaves. The Mosfl1 mutant also had increased sensitivity to elevated temperatures. In CM cultures of the Mosfl1 and pmk1 mutants grown at 30°C, the production of aerial hyphae and melanization were reduced but their growth rate was not altered. When assayed by qRT-PCR, the transcription levels of the MoHSP30 and MoHSP98 genes were reduced 10- and 3-fold, respectively, in the Mosfl1 mutant. SFL1 orthologs are conserved in filamentous ascomycetes but none of them have been functionally characterized in non-Saccharomycetales fungi. MoSfl1 has one putative MAPK docking site and three putative MAPK phosphorylation sites. Therefore, it may be functionally related to Pmk1 in the regulation of invasive growth and stress responses in M. oryzae.
登录
查看更多内容
影响因子：
4.5
作者：
Kim S;Park SY;Kim KS;Rho HS;Chi MH;Choi J;Park J;Kong S;Park J;Goh J;Lee YH
通讯作者：
Lee YH
影响因子：
--
作者：
Bauer, Janine;Wendland, Juergen
通讯作者：
Wendland, Juergen
影响因子：
5.3
作者：
Kim, TS;Lee, SB;Kang, HS
通讯作者：
Kang, HS
影响因子：
2.5
作者：
Galeote, Virginie Ansanay;Alexandre, Herve;Blondin, Bruno
通讯作者：
Blondin, Bruno
影响因子：
11.6
作者：
Adachi, K;Hamer, JE
通讯作者：
Hamer, JE