Fluoro jade-C staining in the assessment of brain injury after deep hypothermia circulatory arrest
Fluoro jade-C staining in the assessment of brain injury after deep hypothermia circulatory arrest
复制标题
荧光玉C染色评估深低温停循环后脑损伤
DOI：
10.1016/j.brainres.2010.11.059
复制
发表时间：
2011-02
期刊：
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
--
作者：
目的探讨FluoroJade-C染色（FluoroJade-C staining，FJC）在深低温停循环（deep hypothermia circulatory arrest，DHCA）脑损伤评估中的应用价值。术后第1天，对脑组织进行灌注固定。取大脑皮质、海马、小脑取样。采用FJC法、HE染色、尼氏染色（NISSL）、末端脱氧核苷酸转移酶-生物素-dUTP缺口末端标记（TUNEL）法检测组织学和病理学变化。对所有切片的组织学评分进行分级。FJC和其他技术之间的比较进行方差分析（ANOVA）根据组织学scores.ResultsAll动物存活的操作。在大脑皮质，HE、NISSL和TUNEL法的FJC比较，p值分别为0.90、0.40、0.16（p>0.05）。海马区FJC与HE、NISSL、TUNEL比较，p值分别为0.12、0.23、0.62（p>0.05）。小脑FJC与HE、NISSL、TUNEL比较，p值分别为0.96、0.77、0.96（p>0.05）。在代表性区域，FJC的结果与TUNEL、NISSL和HE的结果一致。结论FJC是评价DHCA术后脑损伤的一种可靠、简便的方法。
ObjectiveTo evaluate the efficacy of Fluoro Jade-C staining (FJC) in the assessment of brain injury after deep hypothermia circulatory arrest (DHCA).MethodsSix healthy adult miniature male pigs underwent DHCA, the rectal temperature was down to 18°C, circulation was stopped , circulatory arrest was maintained for 60 minutes. On postoperative day 1, perfusion-fixation was performed on brain tissue. Cerebral cortex, hippocampus, cerebellum were taken for sampling. FJC, hematoxylin–eosin staining (HE), nissl staining (NISSL), terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) were performed to detect the histological and pathological changes. Histological scores of all slices were ranked. Comparison between the FJC and other techniques was done by analysis of variance (ANOVA) according to histological scores.ResultsAll animals survived the operation. On the cerebral cortex, in comparison of FJC between HE, NISSL and TUNEL, the p value was 0.90, 0.40, 0.16 respectively (p>0.05). On the hippocampus, the comparison of FJC with HE, NISSL and TUNEL had a p value of 0.12, 0.23, 0.62 respectively (p>0.05). On the cerebellum, in comparing FJC with HE, NISSL and TUNEL, the p value was 0.96, 0.77, 0.96 respectively (p>0.05). On representative regions, the results of FJC were in accordance with that of TUNEL, NISSL and HE. Furthermore, ascertainment of brain injury is easier with FJC.ConclusionFJC is a reliable and convenient method to assess brain injury after DHCA.
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DOI：
10.1053/j.pcsu.2008.01.003
发表时间：
2008
期刊：
Seminars in thoracic and cardiovascular surgery. Pediatric cardiac surgery annual
影响因子：
--
作者：
D. Nelson;D. Andropoulos;C. Fraser
通讯作者：
D. Nelson;D. Andropoulos;C. Fraser
DOI：
10.1016/j.jtcvs.2006.12.056
发表时间：
2007-06-01
影响因子：
6
作者：
Hickey, Edward J.;You, Xiaomang;Ungerleider, Ross M.
通讯作者：
Ungerleider, Ross M.
使用 Fluoro-Jade C 染色观察慢性癫痫小鼠毛果芸香碱模型中神经元死亡的时间进程
影响因子：
2.9
作者：
Wang, Lian;Liu, Yong-Hong;Chen, Liang-Wei
通讯作者：
Chen, Liang-Wei
影响因子：
4.6
作者：
Chen, Jodi;Zimmerman, Robert A.;Jarvik, Gail P.;Nord, Alex S.;Clancy, Robert R.;Wernovsky, Gil;Montenegro, Lisa M.;Hartman, Diane M.;Nicolson, Susan C.;Spray, Thomas L.;Gaynor, J. William;Ichord, Rebecca
通讯作者：
Ichord, Rebecca
影响因子：
2.9
作者：
Schmued, LC;Albertson, C;Slikker, W
通讯作者：
Slikker, W