11S Glycinin Up-Regulated NLRP-3-Induced Pyroptosis by Triggering Reactive Oxygen Species in Porcine Intestinal Epithelial Cells.
11S Glycinin Up-Regulated NLRP-3-Induced Pyroptosis by Triggering Reactive Oxygen Species in Porcine Intestinal Epithelial Cells.
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DOI：
10.3389/fvets.2022.890978
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发表时间：
2022
影响因子：
3.2
通讯作者：
中科院分区：
文献类型：
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作者：
11S glycinin is a major soybean antigenic protein, which induces human and animal allergies. It has been reported to induce intestinal porcine epithelial (IPEC-J2) cell apoptosis, but the role of pyroptosis in 11S glycinin allergies remains unknown. In this study, IPEC-J2 cells were used as an in vitro physiological model to explore the mechanism of 11S glycinin-induced pyroptosis. The cells were incubated with 0, 1, 5, and 10 mg·ml−1 11S glycinin for 24 h. Our results revealed that 11S glycinin significantly inhibited cell proliferation, induced DNA damage, generated active oxygen, decreased mitochondrial membrane potential, and increased the NOD-like receptor protein 3 (NLRP-3) expression of IPEC-J2 cells in a dose-dependent manner. Further, IPEC-J2 cells were transfected with designed sh-NLRP-3 lentivirus to silence NLRP-3. The results showed that 11S glycinin up-regulated the silenced NLRP-3 gene and increased the expression levels of apoptosis-related spot-like protein (ASC), caspase-1, the cleaved gasdermin D, and interleukin-1β. The IPEC-J2 cells showed pyrolysis morphology. Moreover, we revealed that N-acetyl-L-cysteine can significantly inhibit the production of reactive oxygen species and reduce the expression levels of NLRP-3 and the cleaved gasdermin D. Taken together, 11S glycinin up-regulated NLRP-3-induced pyroptosis by triggering reactive oxygen species in IPEC-J2 cells.
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DOI：
10.4049/jimmunol.1102488
发表时间：
2012-03-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Allen IC;Jania CM;Wilson JE;Tekeppe EM;Hua X;Brickey WJ;Kwan M;Koller BH;Tilley SL;Ting JP
通讯作者：
Ting JP
影响因子：
8.7
作者：
Man SM;Karki R;Kanneganti TD
通讯作者：
Kanneganti TD
影响因子：
9.8
作者：
Xiao J;Wang C;Yao JC;Alippe Y;Xu C;Kress D;Civitelli R;Abu-Amer Y;Kanneganti TD;Link DC;Mbalaviele G
通讯作者：
Mbalaviele G
影响因子：
5.4
作者：
Tsai YM;Chiang KH;Hung JY;Chang WA;Lin HP;Shieh JM;Chong IW;Hsu YL
通讯作者：
Hsu YL
影响因子：
2.9
作者：
Gagnon, Christine;Poysa, Vaino;Gleddie, Stephen
通讯作者：
Gleddie, Stephen