Identification and characterization of a novel outer membrane protein receptor required for hemin utilization in Vibrio vulnificus.
Identification and characterization of a novel outer membrane protein receptor required for hemin utilization in Vibrio vulnificus.
复制标题
DOI：
10.1007/s10534-011-9501-y
复制
发表时间：
2012-04
期刊：
影响因子：
3.5
通讯作者：
Crosa, Jorge H.
中科院分区：
文献类型：
--
作者：
Datta, Shreya;Crosa, Jorge H.
创伤弧菌是人类和鱼类败血症和严重伤口感染的原因，其发病机制需要铁。通过外膜受体HupA摄取氯化血红素是其获得铁的许多机制之一。我们在这里报告的一个额外的TonB依赖性氯化血红素受体HvtA的识别，这是需要在与HupA蛋白的最佳氯化血红素利用率。HvtA蛋白是显着同源的其他外膜氯化血红素受体和其表达的反式恢复氯化血红素和血红蛋白的摄取，后者在较弱的程度上，在突变株，这是有缺陷的两个受体。定量RT-PCR结果表明，铁对hvtA基因的转录有调控作用。含有hvtA基因的操纵子与含有氯化血红素受体基因hutR的霍乱弧菌中的操纵子同源，表明hvtA簇从霍乱弧菌垂直传递到创伤弧菌。
Vibrio vulnificus, the cause of septicemia and serious wound infection in humans and fishes, require iron for its pathogenesis. Hemin uptake through the outer membrane receptor, HupA, is one of its many mechanisms by which it acquires iron. We report here the identification of an additional TonB-dependent hemin receptor HvtA, that is needed in conjunction with the HupA protein for optimal hemin utilization. The HvtA protein is significantly homologous to other outer membrane hemin receptors and its expression in trans restored the uptake of hemin and hemoglobin, the latter to a weaker extent, in a mutant strain that was defective in both receptors. Quantitative RT-PCR suggested that transcription of the hvtA gene was iron regulated. The operon containing the hvtA gene is homologous to the operon in V. cholerae containing the hemin receptor gene hutR suggesting a vertical transmission of the hvtA cluster from V. cholerae to V. vulnificus.
登录
查看更多内容
影响因子：
3.6
作者：
Liu M;Naka H;Crosa JH
通讯作者：
Crosa JH
影响因子：
3.1
作者：
Oh, Man Hwan;Lee, Sung Min;Choi, Sang Ho
通讯作者：
Choi, Sang Ho
影响因子：
3.1
作者：
Alice, Alejandro F.;Naka, Hiroaki;Crosa, Jorge H.
通讯作者：
Crosa, Jorge H.
影响因子：
5.6
作者：
STRUYVE, M;MOONS, M;TOMMASSEN, J
通讯作者：
TOMMASSEN, J
影响因子：
3.1
作者：
Lee, JH;Rho, JB;Park, SJ
通讯作者：
Park, SJ