Dengue-specific subviral nanoparticles: design, creation and characterization
Dengue-specific subviral nanoparticles: design, creation and characterization
复制标题
登革热特异性亚病毒纳米颗粒：设计、创建和表征
DOI：
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发表时间：
2013
影响因子：
10.2
通讯作者：
N. Khanna
中科院分区：
文献类型：
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作者：
N. Khetarpal;Ankur Poddar;Satish K Nemani;N. Dhar;Aravind Patil;Priyanka Negi;Ashiya Perween;R. Viswanathan;H. Lünsdorf;Poornima Tyagi;R. Raut;U. Arora;S. Jain;U. Rinas;S. Swaminathan;N. Khanna
背景登革热是当今最重要的虫媒病毒性疾病。新的工具是有效解决登革热问题所必需的。病毒样颗粒（VLP）为开发具有潜在生物医学应用的工具提供了多功能的纳米级平台。从一个潜在的有用的登革热特异性工具的角度来看，登革热病毒包膜蛋白结构域III（EDIII），赋予与病毒型特异性，宿主受体识别和能力，以引发病毒中和antibodies，是一个有吸引力的candidate.MethodsWe已经开发出一种策略，共表达和共纯化肝炎B病毒表面（S）抗原的两种形式：独立和作为与EDIII融合。我们的特点，这些物理和functionals.ResultsThe两种形式的S抗原协会成VLP。这些以功能可及的方式展示EDIII的能力取决于两种形式的S抗原的相对水平。镶嵌VLP融合和未融合的组件在1：4的比例显示最大的功能competitive.ConclusionsVLP武装EDIII可能是潜在的有用的诊断，治疗和预防应用。
BackgroundDengue is today the most significant of arboviral diseases. Novel tools are necessary to effectively address the problem of dengue. Virus-like particles (VLP) offer a versatile nanoscale platform for developing tools with potential biomedical applications. From the perspective of a potentially useful dengue-specific tool, the dengue virus envelope protein domain III (EDIII), endowed with serotype-specificity, host receptor recognition and the capacity to elicit virus-neutralizing antibodies, is an attractive candidate.MethodsWe have developed a strategy to co-express and co-purify Hepatitis B virus surface (S) antigen in two forms: independently and as a fusion with EDIII. We characterized these physically and functionally.ResultsThe two forms of the S antigen associate into VLPs. The ability of these to display EDIII in a functionally accessible manner is dependent upon the relative levels of the two forms of the S antigen. Mosaic VLPs containing the fused and un-fused components in 1:4 ratio displayed maximal functional competence.ConclusionsVLPs armed with EDIII may be potentially useful in diagnostic, therapeutic and prophylactic applications.
影响因子：
7.7
作者：
Yildiz, Ibrahim;Shukla, Sourabh;Steinmetz, Nicole F.
通讯作者：
Steinmetz, Nicole F.