Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina.
Intercellular contact and cargo transfer between Müller glia and to microglia precede apoptotic cell clearance in the developing retina.
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DOI：
10.1242/dev.202407
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发表时间：
2024-01-01
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影响因子：
--
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为了阐明我们对视网膜发育中胶质细胞吞噬作用的理解，我们使用了斑马鱼幼虫的实时成像来提供这一过程的细胞类型特异性分辨率。我们发现，放射状Müller胶质细胞经常参与小胶质细胞的吞噬作用，同时也完成了一个子集的吞噬事件。Müller胶质细胞通过最初的靶细胞接触和吞噬杯形成与垂死细胞积极接触，之后死亡细胞从Müller胶质细胞交换到小胶质细胞。此外，我们发现的证据表明，Müller胶质细胞的材料，可能从最初的Müller细胞吞噬杯，是内化到小胶质细胞隔室。以前没有描述过的穆勒细胞行为，包括货物分裂，摔跤的目标和横向传递的货物邻居。总的来说，我们的工作为神经胶质功能和细胞间相互作用提供了新的见解，这将使未来的工作能够在分子水平上理解这些行为。总结：斑马鱼视网膜发育的实时成像揭示了Müller胶质细胞和小胶质细胞在清除凋亡细胞过程中的细胞间交换。
To clarify our understanding of glial phagocytosis in retinal development, we used real-time imaging of larval zebrafish to provide cell-type specific resolution of this process. We show that radial Müller glia frequently participate in microglial phagocytosis while also completing a subset of phagocytic events. Müller glia actively engage with dying cells through initial target cell contact and phagocytic cup formation, after which an exchange of the dying cell from Müller glia to microglia often takes place. In addition, we find evidence that Müller glia cellular material, possibly from the initial Müller cell phagocytic cup, is internalized into microglial compartments. Previously undescribed Müller cell behaviors were seen, including cargo splitting, wrestling for targets and lateral passing of cargo to neighbors. Collectively, our work provides new insight into glial functions and intercellular interactions, which will allow future work to understand these behaviors on a molecular level. Summary: Real-time imaging of developing zebrafish retinas reveals intercellular exchanges between Müller glial cells and with microglia during the clearance of apoptotic cells.
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DOI：
10.1016/s0165-3806(96)00119-8
发表时间：
1996-11-22
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Egensperger, R;Maslim, J;Stone, J
通讯作者：
Stone, J
影响因子：
6.2
作者：
通讯作者：
--
影响因子：
7.3
作者：
Hsiao, Cheng-Chih;van der Poel, Marlijn;Hamann, Jorg
通讯作者：
Hamann, Jorg
影响因子：
5.3
作者：
Bernardos, Rebecca L.;Barthel, Linda K.;Raymond, Pamela A.
通讯作者：
Raymond, Pamela A.
DOI：
10.1002/dvdy.163
发表时间：
2020-06
期刊：
Developmental dynamics : an official publication of the American Association of Anatomists
影响因子：
--
作者：
Blume ZI;Lambert JM;Lovel AG;Mitchell DM
通讯作者：
Mitchell DM