Carbonization of a stable β-sheet-rich silk protein into a pseudographitic pyroprotein.
Carbonization of a stable β-sheet-rich silk protein into a pseudographitic pyroprotein.
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DOI：
10.1038/ncomms8145
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发表时间：
2015-05-20
影响因子：
16.6
通讯作者：
Jin, Hyoung-Joon
中科院分区：
文献类型：
--
作者：
Cho, Se Youn;Yun, Young Soo;Lee, Sungho;Jang, Dawon;Park, Kyu-Young;Kim, Jae Kyung;Kim, Byung Hoon;Kang, Kisuk;Kaplan, David L.;Jin, Hyoung-Joon
蚕丝蛋白由于其独特的机械性质和有趣的生物功能而引起科学界的极大兴趣。此外，丝蛋白在加热后不会燃烧，而是转化为含碳固体，即热蛋白;一些研究已经确定了最先进技术的潜在碳前体。然而，还没有报道蛋白质碳化的机制。在这里，我们系统地研究了在高于热降解开始的温度下丝蛋白的结构和化学变化。我们发现，通过简单加热到350 °C，β-折叠结构转变为sp2杂化的碳六方结构。在进一步加热至2，800 °C之后，假石墨晶体层生长以形成高度有序的石墨结构。我们的研究结果提供了一种机制的蛋白质的热转变，并证明了一个潜在的策略，设计热蛋白使用一个清洁的系统，无催化剂的水湿过程在体内应用。 丝蛋白的强度和稳定性被认为与其结构中高含量的β-折叠有关。在这里，作者表明，当在高温下加热时，并且高于热降解时，这些β-片层转化为有序的六方石墨结构。
Silk proteins are of great interest to the scientific community owing to their unique mechanical properties and interesting biological functionality. In addition, the silk proteins are not burned out following heating, rather they are transformed into a carbonaceous solid, pyroprotein; several studies have identified potential carbon precursors for state-of-the-art technologies. However, no mechanism for the carbonization of proteins has yet been reported. Here we examine the structural and chemical changes of silk proteins systematically at temperatures above the onset of thermal degradation. We find that the β-sheet structure is transformed into an sp2-hybridized carbon hexagonal structure by simple heating to 350 °C. The pseudographitic crystalline layers grew to form highly ordered graphitic structures following further heating to 2,800 °C. Our results provide a mechanism for the thermal transition of the protein and demonstrate a potential strategy for designing pyroproteins using a clean system with a catalyst-free aqueous wet process for in vivo applications. The strength and stability of silk proteins is thought to be related to the high content of β-sheets within their structures. Here, the authors show that when heated at high temperature, and above that of thermal degradation, these β-sheets are transformed into an ordered hexagonal graphitic structure.
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影响因子：
29.4
作者：
Yun, Young Soo;Cho, Se Youn;Jin, Hyoung-Joon
通讯作者：
Jin, Hyoung-Joon
影响因子：
8.6
作者：
WANG, Y;ALSMEYER, DC;MCCREERY, RL
通讯作者：
MCCREERY, RL
单根天然丝纤维的同步加速器 FTIR 显微光谱分析
影响因子：
6.2
作者：
Ling, Shengjie;Qi, Zeming;Chen, Xin
通讯作者：
Chen, Xin
影响因子：
2.9
作者：
Chen, Xin;Shao, Zhengzhong;Vollrath, Fritz
通讯作者：
Vollrath, Fritz
影响因子：
3.7
作者：
Beatriz Vazquez-Santos, M.;Geissler, Erik;Tascon, Juan M. D.
通讯作者：
Tascon, Juan M. D.