Serotonergic Neurons in the Chick Brainstem Express Various Serotonin Receptor Subfamily Genes.
Serotonergic Neurons in the Chick Brainstem Express Various Serotonin Receptor Subfamily Genes.
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DOI：
10.3389/fphys.2021.815997
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发表时间：
2021
影响因子：
4
通讯作者：
Yamaguchi S
中科院分区：
文献类型：
--
作者：
Fujita T;Aoki N;Mori C;Fujita E;Matsushima T;Homma KJ;Yamaguchi S
5-羟色胺(5-羟色胺，5-HT)是一种系统发育保守的调节性神经递质。在哺乳动物中，5-羟色胺在中枢神经系统的许多精神状态的调节和情绪的处理中起着重要作用。中枢神经系统中的5-羟色胺能神经元，包括中缝背核(DR)和中缝正中核(MR)，在空间上聚集在脑干中，为整个前脑和中脑提供上行神经支配。在DR和MR之间和内部，这些5-羟色胺能神经元具有不同的细胞特征、发育来源、连接性、生理学和相关的行为功能。最近，人们在分子水平上对DR和MR 5-羟色胺能神经元的异质性有了更深入的了解。在鸟类中，与情绪相关的行为被认为是由5-羟色胺系统调节的。然而，鸟类和哺乳动物的中缝核团之间的对应关系以及鸟类5-羟色胺能神经元的细胞异质性却知之甚少。为了进一步了解鸟类5-羟色胺能神经元的异质性，我们用原位杂交的方法对鸡的脑干进行了分子解剖。在本研究中，我们制备了鸡5-羟色胺受体基因5-HTR1A、5-HTR1B、5-HTR1D、5-HTR1E、5-HTR1F、5-HTR2A、5-HTR2B、5-HTR2C、5-HTR3A、5-HTR4、5-HTR5A和5-HTR7的RNA探针。我们发现5-羟色胺受体在雏鸡DR和MR的5-羟色胺神经元中的表达模式可能不同，这表明在雏鸡脑干中DR和MR的5-羟色胺神经元之间和内部存在异质性。我们关于鸟类中缝系统中5-羟色胺能神经元的分子特性的发现将有助于更好地理解鸟类和哺乳动物中缝之间的对应关系。
Serotonin (5-hydroxytryptamine, 5-HT) is a phylogenetically conserved modulatory neurotransmitter. In mammals, 5-HT plays an important role in the regulation of many mental states and the processing of emotions in the central nervous system. Serotonergic neurons in the central nervous system, including the dorsal raphe (DR) and median raphe (MR) nuclei, are spatially clustered in the brainstem and provide ascending innervation to the entire forebrain and midbrain. Both between and within the DR and MR, these serotonergic neurons have different cellular characteristics, developmental origin, connectivity, physiology, and related behavioral functions. Recently, an understanding of the heterogeneity of the DR and MR serotonergic neurons has been developed at the molecular level. In birds, emotion-related behavior is suggested to be modulated by the 5-HT system. However, correspondence between the raphe nuclei of birds and mammals, as well as the cellular heterogeneity in the serotonergic neurons of birds are poorly understood. To further understand the heterogeneity of serotonergic neurons in birds, we performed a molecular dissection of the chick brainstem using in situ hybridization. In this study, we prepared RNA probes for chick orthologs of the following serotonin receptor genes: 5-HTR1A, 5-HTR1B, 5-HTR1D, 5-HTR1E, 5-HTR1F, 5-HTR2A, 5-HTR2B, 5-HTR2C, 5-HTR3A, 5-HTR4, 5-HTR5A, and 5-HTR7. We showed that the expression pattern of 5-HT receptors in the serotonin neurons of chick DR and MR may vary, suggesting heterogeneity among and within the serotonin neurons of the DR and MR in the chick brainstem. Our findings regarding the molecular properties of serotonergic neurons in the bird raphe system will facilitate a good understanding of the correspondence between bird and mammalian raphes.
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DOI：
10.1254/jjp.21.763
发表时间：
1971-01-01
期刊：
Japanese Journal of Pharmacology
影响因子：
--
作者：
IKEDA H;GOTOH J
通讯作者：
GOTOH J
影响因子：
2.7
作者：
Krause, E. Tobias;Kjaer, Joergen B.;Loc Phi van
通讯作者：
Loc Phi van
影响因子：
2.5
作者：
Bonnavion, Patricia;Bernard, Jean-Francois;Fabre, Veronique
通讯作者：
Fabre, Veronique
影响因子：
7.3
作者：
Klein, M. T.;Teitler, M.
通讯作者：
Teitler, M.
影响因子：
4.7
作者：
BRUINVELS, AT;LANDWEHRMEYER, B;PALACIOS, JM
通讯作者：
PALACIOS, JM