B7-1 amplifies the response to interleukin-2-secreting tumor vaccines in vivo, but fails to induce a response by naive cells in vitro.
B7-1 amplifies the response to interleukin-2-secreting tumor vaccines in vivo, but fails to induce a response by naive cells in vitro.
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B7-1 可在体内增强对分泌白细胞介素 2 的肿瘤疫苗的反应，但无法在体外诱导幼稚细胞的反应。
DOI：
10.1089/hum.1995.6.10-1299
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发表时间：
1995
影响因子：
4.2
通讯作者：
Zier,K
中科院分区：
文献类型：
--
作者：
Salvadori,S;Gansbacher,B;Wernick,I;Tirelli,S;Zier,K
用B7 - 1共刺激分子转染亲本和分泌白细胞介素-2（IL-2）的CMS 5肿瘤细胞以增强抗肿瘤应答。转染B7 - 1的CMS5细胞在体内的生长速度比亲本CMS5细胞慢。此外，分泌IL-2水平太低而不能单独引起排斥的肿瘤细胞在用B7 - 1转染后被排斥。为了确定B7 - 1的表达是否能使肿瘤细胞直接激活T细胞，检测了它们刺激T细胞体外功能反应的能力。我们发现B7 - 1+和IL-2分泌型B7 - 1 + CMS5细胞均不能刺激幼稚脾细胞增殖或产生细胞毒性。相比之下，B7 - 1 + CMS 5细胞对引发的T细胞的再刺激导致比亲本CMS 5细胞再刺激后观察到的更强的细胞毒性应答。如果B7 - 1+肿瘤细胞也分泌IL-2，则溶解甚至更高。我们的研究结果表明，共刺激分子的表达可以增强免疫接种IL-2分泌肿瘤细胞产生的反应。此外，它们与以下假设一致，即初始T细胞的抗肿瘤应答的起始可能取决于另一种未鉴定细胞的初始抗原呈递，并且IL-2分泌和/或B7 - 1+肿瘤细胞疫苗的主要作用可能是增强已经引发的细胞的应答。
Parental and interleukin-2 (IL-2)-secreting CMS5 tumor cells were transfected with the B7-1 costimulatory molecule to amplify anti-tumor responses. CMS5 cells transfected with B7-1 grew more slowlyin vivothan did parental CMS5 cells. Moreover, tumor cells secreting levels of IL-2 too low to cause rejection alone were rejected following transfection with B7-1. To determine whether the expression of B7-1 enabled the tumor cells to activate T cells directly, their ability to stimulatein vitrofunctional responses by T cells was examined. We found that neither B7-1+nor IL-2-secreting, B7-1+CMS5 cells stimulated naive spleen cells to proliferate or to become cytotoxic. In contrast, restimulation of primed T cells by B7-1+CMS5 cells resulted in stronger cytotoxicity responses than seen following restimulation by parental CMS5 cells. Lysis was even higher if the B7-1+tumor cells also secreted IL-2. Our results suggest that the expression of costimulatory molecules can augment responses generated by vaccinating with IL-2-secreting tumor cells. Furthermore, they are consistent with the hypothesis that the initiation of an anti-tumor response by naive T cells may depend upon initial antigen presentation by another unidentified cell and that the major action of IL-2-secreting and/or B7-1+tumor cell vaccines might be to potentiate the response of already primed cells.
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影响因子：
64.5
作者：
CHEN, LP;ASHE, S;LINSLEY, PS
通讯作者：
LINSLEY, PS
白介素7诱导CD4+ T细胞依赖性肿瘤排斥。
影响因子：
15.3
作者：
HOCK, H;DORSCH, M;DIAMANTSTEIN, T;BLANKENSTEIN, T
通讯作者：
BLANKENSTEIN, T
使用分泌 IL-2 的肿瘤细胞进行疫苗接种可刺激 IL-2 反应性 T 细胞的产生，并防止无反应性的发生。
影响因子：
6.4
作者：
Zier,KS;Salvadori,S;Cronin,KC;Gansbacher,B
通讯作者：
Gansbacher,B
一种可区分 T 细胞和 B 细胞亚群的单克隆抗体。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Bruce,J;Symington,FW;McKearn,TJ;Sprent,J
通讯作者：
Sprent,J
影响因子：
56.9
作者：
TOWNSEND, SE;ALLISON, JP
通讯作者：
ALLISON, JP