Germ cell differentiation requires Tdrd7-dependent chromatin and transcriptome reprogramming marked by germ plasm relocalization.
Germ cell differentiation requires Tdrd7-dependent chromatin and transcriptome reprogramming marked by germ plasm relocalization.
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DOI：
10.1016/j.devcel.2021.02.007
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发表时间：
2021-03-08
影响因子：
11.8
通讯作者：
Müller F
中科院分区：
文献类型：
--
作者：
D'Orazio FM;Balwierz PJ;González AJ;Guo Y;Hernández-Rodríguez B;Wheatley L;Jasiulewicz A;Hadzhiev Y;Vaquerizas JM;Cairns B;Lenhard B;Müller F
在许多动物模型中，原始生殖细胞（PGC）的发育依赖于母体沉积的种质，这阻止了体细胞的命运。在这里，我们表明，PGC响应于两个不同的阶段，使用两种不同的机制在斑马鱼的种质的监管信息。我们证明，原始生殖细胞开始合子基因组激活与体细胞胚泡没有明显的差异，在转录和染色质开放。出乎意料的是，PGC和体胚泡都激活生殖细胞特异性基因，这些基因仅在PGC中通过细胞质种质决定簇稳定。PGC迁移过程中种质的解聚核周再定位受种质决定子Tdrd7的调控，并与PGC和体细胞转录组之间的显著差异相关。这种转录差异依赖于PGC特异性顺式调控元件，其特征在于启动子近端分布。我们发现，Tdrd7依赖的染色质可及性的重新配置是需要的PGC的命运，但不是PGC迁移的阐述。没有证据表明斑马鱼PGCs中的转录激活延迟ZGA期间种质相关的转录后趋异表观遗传重编程标志着PGC迁移的开始PGC中的表观遗传重编程依赖于Tdrd 7，与种质重定位相结合D 'Orazio et al.显示了斑马鱼原始生殖细胞（PGCs）中合子基因组激活发生在两个阶段。PGCs和体细胞同时激活基因组，显示出相似的转录和染色质景观。分歧发生在PGC迁移过程中，当PGC特异性染色质景观耦合到Tdrd7a依赖的核周种质再定位。
In many animal models, primordial germ cell (PGC) development depends on maternally deposited germ plasm, which prevents somatic cell fate. Here, we show that PGCs respond to regulatory information from the germ plasm in two distinct phases using two distinct mechanisms in zebrafish. We demonstrate that PGCs commence zygotic genome activation together with the somatic blastocysts with no demonstrable differences in transcriptional and chromatin opening. Unexpectedly, both PGC and somatic blastocysts activate germ-cell-specific genes, which are only stabilized in PGCs by cytoplasmic germ plasm determinants. Disaggregated perinuclear relocalization of germ plasm during PGC migration is regulated by the germ plasm determinant Tdrd7 and is coupled to dramatic divergence between PGC and somatic transcriptomes. This transcriptional divergence relies on PGC-specific cis-regulatory elements characterized by promoter-proximal distribution. We show that Tdrd7-dependent reconfiguration of chromatin accessibility is required for elaboration of PGC fate but not for PGC migration. No evidence for transcriptional activation delay in zebrafish PGCs Germ-plasm-associated post-transcriptional divergence during ZGA Epigenetic reprogramming marks onset of PGC migration Epigenetic reprogramming in PGCs relies on Tdrd7, coupled to germ plasm relocalization D’Orazio et al. show that zygotic genome activation in zebrafish primordial germ cells (PGCs) occurs in two phases. PGCs and somatic cells activate the genome concurrently, showing similar transcriptional and chromatin landscapes. Divergence occurs during PGC migration, when a PGC-specific chromatin landscape is coupled to Tdrd7a-dependent perinuclear germ plasm relocalization.
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