Atrial natriuretic factor in human blood.
Atrial natriuretic factor in human blood.
复制标题
人体血液中的心房钠尿因子。
DOI：
10.1172/jci112159
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
F. Takaku
中科院分区：
文献类型：
--
作者：
T. Yamaji;M. Ishibashi;F. Takaku
为了确定心房钠尿因子 (ANF) 是否是男性的循环激素，开发了一种适合评估人血浆中 ANF 的放射免疫测定法，并对血浆 ANF 的性质进行了表征。在放射免疫测定之前，在 ANF 抗体偶联琼脂糖柱上通过亲和层析提取血浆 ANF。当用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析血浆ANF提取物时，对洗脱的凝胶切片进行放射免疫分析，几乎所有的ANF活性都在3,000mol-wt区域，而在人心房组织提取物中观察到三个ANF峰，其分子量分别对应于14,000、6,000和3,000。心房组织提取物的反相高效液相色谱解析了多种形式的 ANF。相比之下，在人血浆提取物中观察到一个主峰，其保留时间与合成的人α-心房钠尿多肽的保留时间一致。当 45 分钟内将 500 毫升 0.9% 盐水注入六名健康受试者时，ANF 血浆水平明显升高。输注后 75 分钟，平均血浆 ANF 浓度从基线（23.0 +/- 2.5 pg/ml，平均值 +/- SEM，n = 6）上升至峰值（41.8 +/- 4.9 pg/ml，平均值 +/- SEM）。另一方面，对照组的血浆 ANF 没有显着变化。这些结果表明，ANF 是男性的一种循环激素，是响应等渗容量扩张而分泌的。
To determine whether atrial natriuretic factor (ANF) is a circulating hormone in men, a radioimmunoassay suitable for the estimation of ANF in human plasma was developed and the nature of plasma ANF was characterized. Plasma ANF was extracted before radioimmunoassay by affinity chromatography on a column of ANF antibody-coupled agarose. When plasma ANF extract was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with the radioimmunoassay of the eluted gel slices for ANF, almost all of the ANF activities ran in the 3,000-mol-wt area, while three peaks of ANF were observed in human atrial tissue extract, molecular weights of which corresponded to 14,000, 6,000, and 3,000, respectively. Reversed-phase high performance liquid chromatography of atrial tissue extract resolved multiple forms of ANF. In contrast, one major peak was observed in human plasma extract, and its retention time coincided with that of synthetic human alpha-atrial natriuretic polypeptide. When 500 ml of 0.9% saline was infused into six healthy subjects over 45 min, plasma levels of ANF were unequivocally elevated. The mean plasma ANF concentrations rose from the baseline (23.0 +/- 2.5 pg/ml, mean +/- SEM, n = 6) to the peak (41.8 +/- 4.9 pg/ml, mean +/- SEM) at 75 min postinfusion. No significant change in plasma ANF, on the other hand, was found in the control group. These results suggest that ANF is a circulating hormone in men and is secreted in response to isotonic volume expansion.
大鼠下丘脑、心房和血浆中的心房钠尿因子：通过特异性放射免疫测定法测定。
DOI：
10.1016/0006-291x(84)91606-1
发表时间：
1984
影响因子：
3.1
作者：
Tanaka,I;Misono,KS;Inagami,T
通讯作者：
Inagami,T