Molecular cDNA cloning and tissue distribution of mRNA encoding a novel ATP-binding cassette (ABC) half-transporter.
Molecular cDNA cloning and tissue distribution of mRNA encoding a novel ATP-binding cassette (ABC) half-transporter.
复制标题
编码新型 ATP 结合盒 (ABC) 半转运蛋白的 mRNA 的分子 cDNA 克隆和组织分布。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
G. F. Kahl
中科院分区：
文献类型：
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作者：
K. Hirsch‐Ernst;S. Gaini;B. Ernst;C. Schmitz;S. Blume;G. F. Kahl
大多数属于ATP结合盒（ABC）超家族的蛋白质催化底物跨生物膜的转运。采用逆转录-PCR方法与简并引物，我们已经确定了全长cDNA从大鼠肝细胞编码一种新的ABC转运蛋白称为umat（遍在表达的哺乳动物ABC半转运蛋白）。推导的836个氨基酸的序列包括一个N-末端膜锚结构域和一个保守的C-末端核苷酸结合折叠，指定umat作为ABC半转运蛋白。虽然前250个氨基酸位置与其他ABC转运蛋白高度不同，但沿着蛋白质的其余部分沿着存在保守残基簇。最大的序列相似性观察与裂殖酵母重金属耐受蛋白hmt 1（44.5%的626个氨基酸重叠的同一性）。Umat mRNA在所有分析的组织中表达，在睾丸中最丰富。在培养的原代大鼠肝细胞中大量umat mRNA表达表明，肝细胞培养物应代表用于调查umat功能和调节的适当模型。
The majority of proteins belonging to the ATP-binding cassette (ABC) superfamily catalyzes translocation of substrates across biological membranes. Employing a reverse transcription-PCR approach with degenerate primers, we have identified a full-length cDNA from rat hepatocytes encoding a novel ABC transporter termed umat (ubiquitously expressed mammalian ABC half-transporter). The deduced sequence of 836 amino acids comprises an N-terminal membrane anchor domain and a single conserved C-terminal nucleotide binding fold, specifying umat as an ABC half-transporter. While the first 250 amino acid positions are highly divergent from other ABC transporters, clusters of conserved residues are evident along the rest of the protein. The greatest sequence similarity was observed with the fission yeast heavy metal tolerance protein hmt1 (44.5% identity in a 626-amino-acid overlap). Umat mRNA, expressed in all tissues analyzed, was most abundant in testis. Substantial umat mRNA expression in cultured primary rat hepatocytes suggests that hepatocyte cultures should represent an adequate model for investigation of umat function and regulation.
影响因子：
11.4
作者：
LEIGHTON, J;SCHATZ, G
通讯作者：
SCHATZ, G
苯巴比妥诱导大鼠肝细胞色素 P-450b 和 P-450e。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Omiecinski,CJ;WalzJr,FG;Vlasuk,GP
通讯作者：
Vlasuk,GP