Adenovirus transduction to express human ACE2 causes obesity-specific morbidity in mice, impeding studies on the effect of host nutritional status on SARS-CoV-2 pathogenesis.
Adenovirus transduction to express human ACE2 causes obesity-specific morbidity in mice, impeding studies on the effect of host nutritional status on SARS-CoV-2 pathogenesis.
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表达人ACE2的腺病毒转导导致小鼠肥胖特异性发病率，这阻碍了宿主营养状况对SARS-COV-2发病机理的影响的研究。
DOI：
10.1016/j.virol.2021.08.014
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发表时间：
2021-11
期刊：
影响因子：
3.7
通讯作者：
Lucarelli JW
中科院分区：
文献类型：
--
作者：
Rai P;Chuong C;LeRoith T;Smyth JW;Panov J;Levi M;Kehn-Hall K;Duggal NK;Lucarelli JW
新冠肺炎疫情使全球经济陷入瘫痪，并导致全球数以百万计的人死亡。患有肥胖、糖尿病和高血压等并存疾病的人患严重新冠肺炎疾病的风险增加。这是压倒性的担忧，因为42%的美国人肥胖，30%的人患有糖尿病前期，9.4%的人患有临床糖尿病。在这里，我们使用一个公认的饮食诱导肥胖的小鼠模型，研究了肥胖对SARS-CoV-2感染后疾病严重性的影响。用复制缺陷型腺病毒转导ACE2表达的饮食诱导肥胖和瘦肉对照C57BL/6-N小鼠，感染SARS-CoV-2，监测肺病理、病毒滴度和细胞因子表达。感染SARS-CoV-2的AdV转导的瘦肉组和肥胖组在组织病理学和病毒复制方面没有显著差异，但某些细胞因子在感染肥胖组的表达明显高于瘦肉组。值得注意的是，在使用腺病毒载体治疗的肥胖小鼠中，观察到显著的体重减轻，与SARS-CoV-2感染无关，这表明该载体导致了肥胖相关的发病率。这些数据表明，这里和其他地方描述的腺病毒转导的SARS-CoV-2感染小鼠模型可能不适合进行营养研究。
The COVID-19 pandemic has paralyzed the global economy and resulted in millions of deaths globally. People with co-morbidities like obesity, diabetes and hypertension are at an increased risk for severe COVID-19 illness. This is of overwhelming concern because 42% of Americans are obese, 30% are pre-diabetic and 9.4% have clinical diabetes. Here, we investigated the effect of obesity on disease severity following SARS-CoV-2 infection using a well-established mouse model of diet-induced obesity. Diet-induced obese and lean control C57BL/6 N mice, transduced for ACE2 expression using replication-defective adenovirus, were infected with SARS-CoV-2, and monitored for lung pathology, viral titers, and cytokine expression. No significant differences in tissue pathology or viral replication was observed between AdV transduced lean and obese groups, infected with SARS-CoV-2, but certain cytokines were expressed more significantly in infected obese mice compared to the lean ones. Notably, significant weight loss was observed in obese mice treated with the adenovirus vector, independent of SARS-CoV-2 infection, suggesting an obesity-dependent morbidity induced by the vector. These data indicate that the adenovirus-transduced mouse model of SARS-CoV-2 infection, as described here and elsewhere, may be inappropriate for nutrition studies.
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影响因子：
5
作者：
Gadotti AC;de Castro Deus M;Telles JP;Wind R;Goes M;Garcia Charello Ossoski R;de Padua AM;de Noronha L;Moreno-Amaral A;Baena CP;Tuon FF
通讯作者：
Tuon FF
影响因子：
6.4
作者：
Alagaili AN;Briese T;Mishra N;Kapoor V;Sameroff SC;Burbelo PD;de Wit E;Munster VJ;Hensley LE;Zalmout IS;Kapoor A;Epstein JH;Karesh WB;Daszak P;Mohammed OB;Lipkin WI
通讯作者：
Lipkin WI
DOI：
10.1038/nrmicro.2016.81
发表时间：
2016-08
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
de Wit E;van Doremalen N;Falzarano D;Munster VJ
通讯作者：
Munster VJ
危及生命的Covid-19患者中针对I型IFN的自身抗体。
DOI：
10.1126/science.abd4585
发表时间：
2020-10-23
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bastard P;Rosen LB;Zhang Q;Michailidis E;Hoffmann HH;Zhang Y;Dorgham K;Philippot Q;Rosain J;Béziat V;Manry J;Shaw E;Haljasmägi L;Peterson P;Lorenzo L;Bizien L;Trouillet-Assant S;Dobbs K;de Jesus AA;Belot A;Kallaste A;Catherinot E;Tandjaoui-Lambiotte Y;Le Pen J;Kerner G;Bigio B;Seeleuthner Y;Yang R;Bolze A;Spaan AN;Delmonte OM;Abers MS;Aiuti A;Casari G;Lampasona V;Piemonti L;Ciceri F;Bilguvar K;Lifton RP;Vasse M;Smadja DM;Migaud M;Hadjadj J;Terrier B;Duffy D;Quintana-Murci L;van de Beek D;Roussel L;Vinh DC;Tangye SG;Haerynck F;Dalmau D;Martinez-Picado J;Brodin P;Nussenzweig MC;Boisson-Dupuis S;Rodríguez-Gallego C;Vogt G;Mogensen TH;Oler AJ;Gu J;Burbelo PD;Cohen JI;Biondi A;Bettini LR;D'Angio M;Bonfanti P;Rossignol P;Mayaux J;Rieux-Laucat F;Husebye ES;Fusco F;Ursini MV;Imberti L;Sottini A;Paghera S;Quiros-Roldan E;Rossi C;Castagnoli R;Montagna D;Licari A;Marseglia GL;Duval X;Ghosn J;HGID Lab;NIAID-USUHS Immune Response to COVID Group;COVID Clinicians;COVID-STORM Clinicians;Imagine COVID Group;French COVID Cohort Study Group;Milieu Intérieur Consortium;CoV-Contact Cohort;Amsterdam UMC Covid-19 Biobank;COVID Human Genetic Effort;Tsang JS;Goldbach-Mansky R;Kisand K;Lionakis MS;Puel A;Zhang SY;Holland SM;Gorochov G;Jouanguy E;Rice CM;Cobat A;Notarangelo LD;Abel L;Su HC;Casanova JL
通讯作者：
Casanova JL
影响因子：
46.9
作者：
Chua, Robert Lorenz;Lukassen, Soeren;Eils, Roland
通讯作者：
Eils, Roland