Tensile mechanical changes in the Achilles tendon due to Insertional Achilles tendinopathy.
Tensile mechanical changes in the Achilles tendon due to Insertional Achilles tendinopathy.
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DOI：
10.1016/j.jmbbm.2020.104031
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发表时间：
2020-12
影响因子：
3.9
通讯作者：
Buckley MR
中科院分区：
文献类型：
--
作者：
Bah I;Fernandes NRJ;Chimenti RL;Ketz J;Flemister AS;Buckley MR
插入性跟腱病（IAT）是一种疼痛的疾病，非手术治疗具有挑战性。尽管先前的研究已经表征了受IAT影响的组织的大体组织学特征、体内应变模式和横向压缩机械特性，但是尚不知道IAT如何影响跟腱附着点沿着轴向/纵向方向（即，沿着载荷的主要方向）。为了解决这一知识缺口，本研究的目的是：1）应用离体力学测试、非线性弹性分析和准线性粘弹性（QLV）分析，比较有和没有IAT的个体的跟腱止点的轴向拉伸力学性能; 2）使用生化分析和二次谐波发生（SHG）成像来评估IAT诱导的结构和组成变化，以帮助解释IAT相关的拉伸力学变化。跟腱止点的组织取自健康供体和接受IAT清创手术的患者。使用沿轴向施加的单轴拉伸（应力松弛）测试对组织样本进行机械测试。供体样本的子集用于SHG成像和生化分析。应力松弛试验的线性和非线性弹性分析表明，与健康对照组相比，IAT样本没有显着的拉伸力学变化。然而，SHG分析表明，纤维胶原蛋白是显着更混乱的IAT组织相比，健康对照组，和生化分析表明，硫酸化糖胺聚糖（sGAG）含量和水含量较高的IAT标本。总的来说，这些研究结果表明，IAT的保守干预措施应针对超微结构组织的恢复，降低GAG含量，并降低对横向压缩应变的抵抗力。
Insertional Achilles tendinopathy (IAT) is a painful condition that is challenging to treat non-operatively. Although previous studies have characterized the gross histological features, in vivo strain patterns and transverse compressive mechanical properties of tissue affected by IAT, it is not known how IAT impacts the tensile mechanical properties of the Achilles tendon insertion along the axial/longitudinal direction (i.e., along the predominant direction of loading). To address this knowledge gap, the objectives of this study were to 1) apply ex vivo mechanical testing, nonlinear elastic analysis and quasilinear viscoelastic (QLV) analysis to compare the axial tensile mechanical properties of the Achilles tendon insertion in individuals with and without IAT; and 2) use biochemical analysis and second harmonic generation (SHG) imaging to assess structural and compositional changes induced by IAT in order to help explain IAT-associated tensile mechanical changes. Tissue from the Achilles tendon insertion was acquired from healthy donors and from patients undergoing debridement surgery for IAT. Tissue specimens were mechanically tested using a uniaxial tensile (stress relaxation) test applied in the axial direction. A subset of the donor specimens was used for SHG imaging and biochemical analysis. Linear and non-linear elastic analyses of the stress relaxation tests showed no significant tensile mechanical changes in IAT specimens compared to healthy controls. However, SHG analysis showed that fibrillar collagen was significantly more disorganized in IAT tissue as compared with healthy controls, and biochemical analysis showed that sulfated glycosaminoglycan (sGAG) content and water content were higher in IAT specimens. Collectively, these findings suggest that conservative interventions for IAT should target restoration of ultrastructural organization, reduced GAG content, and reduced resistance to transverse compressive strain.
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影响因子：
1.8
作者：
Hoenig, JM;Heisey, DM
通讯作者：
Heisey, DM
DOI：
10.1002/jor.23338
发表时间：
2017-04
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Chimenti RL;Bucklin M;Kelly M;Ketz J;Flemister AS;Richards MS;Buckley MR
通讯作者：
Buckley MR
影响因子：
3.7
作者：
de Sá A;Hart DA;Khan K;Scott A
通讯作者：
Scott A
DOI：
10.1016/j.apmr.2015.12.009
发表时间：
2016-07-01
影响因子：
4.3
作者：
Chimenti, Ruth L.;Chimenti, Peter C.;Flemister, A. Samuel
通讯作者：
Flemister, A. Samuel
DOI：
10.1115/1.4023234
发表时间：
2013-02-01
影响因子：
1.7
作者：
Connizzo, Brianne K.;Sarver, Joseph J.;Soslowsky, Louis J.
通讯作者：
Soslowsky, Louis J.