Probing the Electron Transfer between iLOV Protein and Ag Nanoparticles
Probing the Electron Transfer between iLOV Protein and Ag Nanoparticles
复制标题
探究 iLOV 蛋白和银纳米粒子之间的电子转移
DOI：
10.3390/molecules25112544
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发表时间：
2020-05
期刊：
影响因子：
4.6
通讯作者：
Lijun Guo
中科院分区：
文献类型：
--
作者：
Xia Ran;Qianqian Zhang;Yu Zhang;Jin Chen;Zhongran Wei;Yulu He;Lijun Guo
Nanomaterials have been widely used in biomedical sciences; however, the mechanism of interaction between nanoparticles and biomolecules is still not fully understood. In the present study, we report the interaction mechanism between differently sized Ag nanoparticles and the improved light-oxygen-voltage (iLOV) protein. The steady-state and time-resolved fluorescence results demonstrated that the fluorescence intensity and lifetime of the iLOV protein decreased upon its adsorption onto Ag nanoparticles, and this decrease was dependent upon nanoparticle size. Further, we showed that the decrease of fluorescence intensity and lifetime arose from electron transfer between iLOV and Ag nanoparticles. Moreover, through point mutation and controlled experimentation, we demonstrated for the first time that electron transfer between iLOV and Ag nanoparticles is mediated by the tryptophan residue in the iLOV protein. These results are of great importance in revealing the function of iLOV protein as it applies to biomolecular sensors, the field of nano-photonics, and the interaction mechanism between the protein and nanoparticles.
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DOI：
10.3390/ma11040603
发表时间：
2018-04-13
期刊：
Materials (Basel, Switzerland)
影响因子：
--
作者：
La Spada L;Vegni L
通讯作者：
Vegni L
影响因子：
9.5
作者：
Mondal, Sanjoy;Rana, Utpal;Malik, Sudip
通讯作者：
Malik, Sudip
影响因子：
2.9
作者：
Liu, T;Zhong, J;Matsuda, N
通讯作者：
Matsuda, N
影响因子：
5.8
作者：
Das, Manash R.;Sarma, Rupak K.;Sengupta, Pinaki
通讯作者：
Sengupta, Pinaki
影响因子：
2.8
作者：
Y. Ravikumar;S. Nadarajan;Chong-soon Lee;J. Rhee;Hyung-Don Yun
通讯作者：
Y. Ravikumar;S. Nadarajan;Chong-soon Lee;J. Rhee;Hyung-Don Yun