Accelerated neural differentiation of mouse embryonic stem cells on aligned GYIGSR-functionalized nanofibers.
Accelerated neural differentiation of mouse embryonic stem cells on aligned GYIGSR-functionalized nanofibers.
复制标题
DOI：
10.1016/j.actbio.2018.05.052
复制
发表时间：
2018-07-15
影响因子：
9.7
通讯作者：
Willits RK
中科院分区：
文献类型：
--
作者：
Silantyeva EA;Nasir W;Carpenter J;Manahan O;Becker ML;Willits RK
胚胎干细胞培养的底物以定义不清的异种、全蛋白或细胞成分为典型，这些成分难以生成、表征和概括。本研究利用Gly-Tyr-Ile-Gly-Ser-Arg （GYIGSR）多肽功能化聚（ԑ-己内酯）（PCL）纳米纤维定向构建明确的支架，用于加速D3小鼠胚胎干细胞（mESCs）的神经谱系承诺和分化。基因表达趋势和免疫细胞化学分析与层粘连蛋白涂层玻璃相似，表明其分化进程比D3 mESCs更早。此外，gyigsr功能化的纳米纤维底物在早期时间点增加了神经祖细胞标记物Sox1和神经细胞标记物Tubb3、Cdh2、Syp的基因表达。此外，神经突的引导方向与纤维方向平行。在此，我们展示了一种定义明确、无异种功能的纳米纤维支架的制造，并展示了它作为目前用于体外干细胞神经分化的异种和复杂基质的替代品的用途。
Substrates for embryonic stem cell culture are typified by poorly defined xenogenic, whole proteins or cellular components that are difficult and expensive to generate, characterize, and recapitulate. Herein, the generation of well-defined scaffolds of Gly-Tyr-Ile-Gly-Ser-Arg (GYIGSR) peptide-functionalized poly(ԑ-caprolactone) (PCL) aligned nanofibers are used to accelerate the neural lineage commitment and differentiation of D3 mouse embryonic stem cells (mESCs). Gene expression trends and immunocytochemistry analysis were similar to laminin-coated glass, and indicated an earlier differentiation progression than D3 mESCs on laminin. Further, GYIGSR-functionalized nanofiber substrates yielded an increased gene expression of Sox1, a neural progenitor cell marker, and Tubb3, Cdh2, Syp, neuronal cell markers, at early time points. In addition, guidance of neurites was found to parallel the fiber direction. Herein, we demonstrate the fabrication of a well-defined, xeno-free functional nanofiber scaffold and demonstrates its use as a surrogate for xenogenic and complex matrixes currently used for the neural differentiation of stem cells ex vivo.
登录
查看更多内容
影响因子：
14
作者：
Callahan, Laura A. Smith;Xie, Sibai;Becker, Matthew L.
通讯作者：
Becker, Matthew L.
影响因子：
2.9
作者：
GRAF, J;OGLE, RC;KLEINMAN, HK
通讯作者：
KLEINMAN, HK
影响因子：
5.2
作者：
de Almeida, Gabriela;Townsend, Lisa C.;Bertozzi, Carolyn R.
通讯作者：
Bertozzi, Carolyn R.
影响因子：
3.2
作者：
Beatty, Kimberly E.;Fisk, John D.;Smart, Brian P.;Lu, Ying Ying;Szychowski, Janek;Hangauer, Matthew J.;Baskin, Jeremy M.;Bertozzi, Carolyn R.;Tirrell, David A.
通讯作者：
Tirrell, David A.
影响因子：
14
作者：
Brafman, David A.;Chang, Chien W.;Fernandez, Antonio;Willert, Karl;Varghese, Shyni;Chien, Shu
通讯作者：
Chien, Shu