Studies on the apoptosis inducing and cell cycle regulatory effect of Cuscuta reflexa Roxb chloroform extract on human hepatocellular carcinoma cell line, Hep 3B
Studies on the apoptosis inducing and cell cycle regulatory effect of Cuscuta reflexa Roxb chloroform extract on human hepatocellular carcinoma cell line, Hep 3B
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菟丝子氯仿提取物对人肝癌细胞Hep 3B的凋亡诱导及细胞周期调控作用研究
DOI：
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
V. Asha
中科院分区：
文献类型：
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作者：
R. J. Praseeja;P. Sreejith;V. Asha
摘要本实验室的初步研究表明，反枝菟丝子对人肝癌细胞株Hep 3B具有一定的抗肝癌活性。本研究的目的是详细阐述C. reflexa对HCC细胞系的作用。C. reflexa（CRCE）以剂量和时间依赖性方式降低Hep 3B细胞的增殖，而对非肝细胞细胞系HEK 293和RAW 264.7没有显示出太多的细胞毒性作用。C.通过DNA片段化、Annexin V-FITC凋亡检测、PARP裂解、Caspase激活等实验证明，reflexa可诱导Hep 3B细胞凋亡。它还引起G1期阻滞，与凋亡诱导相关。还通过TLC分离CRCE中存在的活性级分。工业相关性。肝细胞癌（HCC）由于其在世界范围内的发病率不断增加且生存率低而受到临床广泛关注。这种疾病目前还没有完全治愈的方法。C. reflexa在民族医学上用于治疗各种疾病，尤其是黄疸。本研究观察了CRCE对肝癌细胞系Hep 3B的凋亡诱导作用及相关的细胞周期调控作用。结果表明，CRCE可诱导Hep 3B细胞凋亡，并呈剂量和时间依赖性，其凋亡途径主要是线粒体凋亡途径。CRCE是进一步开发为抗肝癌药物的潜在候选者。分析了CRCE的HPTLC指纹图谱，并分离了有效部位。需要进一步的研究来鉴定和表征分离的活性组分，用于开发活性抗肝癌药物。关键词肝细胞癌; Hep 3B;反枝菟丝子;细胞凋亡;细胞周期阻滞
Summary. Preliminary studies in our lab showed the anti-hepatocellular carcinoma (HCC) activity of Cuscuta reflexa in Hep 3B cell line. This study aims to detail the mechanism behind the anti-HCC effect of C. reflexa on HCC cell line. The chloroform extract of C. reflexa (CRCE) reduced the proliferation of Hep 3B cells in a dose and time dependent manner without showing much cytotoxic effect on non-hepatocyte cell lines, HEK 293 and RAW 264.7. C. reflexa induced apoptosis in Hep 3B cells as evidenced by DNA fragmentation, Annexin V-FITC apoptotic assay, PARP cleavage, Caspase activation etc. Treatment with this extract significantly increased the percentage of apoptotic cells. It also caused G1 arrest, associated with apoptotic induction. The active fractions present in CRCE were also isolated by TLC. Industrial relevance. Hepatocellular carcinoma (HCC) has gained major clinical interest because of its worldwide increasing incidence and carries a poor survival rate. A complete cure for this disease is not available today. C. reflexa is ethnomedically used for the treatment of various diseases especially jaundice. In this study we have evaluated the apoptosis inducing and associated cell cycle regulatory effect of CRCE in HCC cell line (Hep 3B). Results showed that CRCE is able to induce apoptosis in Hep 3B cells in a dose and time dependent manner through the intrinsic mitochondrial apoptotic pathway. CRCE is a potential candidate for further development as an anti-HCC drug. The HPTLC fingerprint profile of CRCE was analyzed and the active fraction was isolated. Further studies are needed to identify and characterize the isolated active fraction for the development of active anti-HCC drug. Keywords. Hepatocellular carcinoma; Hep 3B; Cuscuta reflexa ; Apoptosis; Cell cycle arrest
影响因子：
10.5
作者：
Villanueva A;Minguez B;Forner A;Reig M;Llovet JM
通讯作者：
Llovet JM
影响因子：
4
作者：
Rao, L;White, E
通讯作者：
White, E