Fixation of Bovine Pericardium-Based Tissue Biomaterial with Irreversible Chemistry Improves Biochemical and Biomechanical Properties.
Fixation of Bovine Pericardium-Based Tissue Biomaterial with Irreversible Chemistry Improves Biochemical and Biomechanical Properties.
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DOI：
10.1007/s12265-017-9733-5
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发表时间：
2017-04
影响因子：
3.4
通讯作者：
Vyavahare N
中科院分区：
文献类型：
--
作者：
Tam H;Zhang W;Infante D;Parchment N;Sacks M;Vyavahare N
生物假体心脏瓣膜 (BHV) 源自戊二醛交联 (GLUT) 猪主动脉瓣叶或牛心包 (BP)，用于替代有缺陷的心脏瓣膜。然而，由于钙化和/或进行性结构退化，瓣膜可能在 12-15 年内发生故障。我们提出了一种新的制造方法，利用碳二亚胺、三硫酸新霉素和五没食子酰葡萄糖交联化学（TRI）来更好地稳定 BP 的细胞外基质。我们证明 TRI 处理的 BP 比 GLUT 处理的 BP 更合规。当受到诱导静态应变时，经过 GLUT 处理的 BP 表现出永久的几何变形和表观机械性能的完全改变。另一方面，TRI BP 没有表现出这种永久的几何变形或表观机械性能的显着变化。当 TRI BP 植入幼年大鼠皮下长达 30 天时，还表现出更好的体外抗酶降解性和体内钙化能力。
Bioprosthetic heart valves (BHVs), derived from glutaraldehyde crosslinked (GLUT) porcine aortic valve leaflets or bovine pericardium (BP), are used to replace defective heart valves. However, valve failure can occur within 12–15 years due to calcification and/or progressive structural degeneration. We present a novel fabrication method that utilizes carbodiimide, neomycin trisulfate, and pentagalloyl glucose crosslinking chemistry (TRI) to better stabilize the extracellular matrix of BP. We demonstrate that TRI-treated BP is more compliant than GLUT-treated BP. GLUT-treated BP exhibited permanent geometric deformation and complete alteration of apparent mechanical properties when subjected to induced static strain. TRI BP, on the other hand, did not exhibit such permanent geometric deformations or significant alterations of apparent mechanical properties. TRI BP also exhibited better resistance to enzymatic degradation in vitro and calcification in vivo when implanted subcutaneously in juvenile rats for up to 30 days.
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影响因子：
4.6
作者：
SCOTT, M;VESELY, I
通讯作者：
VESELY, I
影响因子：
14
作者：
Damink, LHHO;Dijkstra, PJ;Feijen, J
通讯作者：
Feijen, J
影响因子：
14
作者：
Chow, James P.;Simionescu, Dan T.;Simionescu, Agneta
通讯作者：
Simionescu, Agneta
DOI：
10.1002/jbm.10293
发表时间：
2002-12-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Sacks, MS;Schoen, FJ
通讯作者：
Schoen, FJ
影响因子：
3.8
作者：
Grashow, Jonathan S.;Sacks, Michael S.;Yoganathan, Ajit P.
通讯作者：
Yoganathan, Ajit P.