Identification of elevated transcripts in a Trichoderma reesei strain expressing a chimeric transcription activator using suppression subtractive hybridization
Identification of elevated transcripts in a Trichoderma reesei strain expressing a chimeric transcription activator using suppression subtractive hybridization
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使用抑制消减杂交鉴定表达嵌合转录激活剂的里氏木霉菌株中升高的转录物
DOI：
10.1007/s11274-009-9993-6
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发表时间：
2009-02
期刊：
影响因子：
--
通讯作者：
Dong, Zhiyang
中科院分区：
文献类型：
--
作者：
Su, Xiaoyun;Chu, Xin;Dong, Zhiyang
为了了解两个转录抑制因子Cre1和ACEI对其下游基因的影响，采用了一种新的策略，即构建了一个含有Cre1和ACEI的DNA结合域和ACEII的效应结构域的嵌合转录激活因子的质粒，并将其转化到里氏木霉中。利用抑制消减杂交技术，在转化子中检测到19个高表达的转录本。它们都与CRE1(5‘-SYGGRG-3’)和ACEI(5‘-AGGCA-3’)有一致的结合基序，其中7个启动子的启动子具有最具代表性的ACEI结合基序(5‘-AGGCAAA-3’)。在体外，Cre1可以与所有基因的启动子结合，而ACEI可以与7个基因中的6个基因的启动子结合。这些结果提供了一组可能与Cre1或ACEI相关的基因的初步概述。这是了解这两个抑制子在细胞途径中的调节作用的第一步，这将有助于里氏木霉菌株的改良。
To know how the two transcription repressors Cre1 and ACEI affect their downstream genes, a new strategy was employed in which a plasmid encoding a chimeric transcription activator containing the DNA binding domains from Cre1 and ACEI and the effector domain from ACEII was constructed and transformed into Trichoderma reesei. Nineteen elevated transcripts were identified in the transformant against its parent strain using suppression subtractive hybridization. All of them had the consensus binding motif for Cre1 (5′-SYGGRG-3′) and ACEI (5′-AGGCA-3′), among which seven had the most representative binding motif (5′-AGGCAAA-3′) for ACEI in their 1,000-bp promoters. Cre1 could bind to the promoters of all the genes while ACEI could bind to the promoters of six out of the seven in vitro. The results provide a primary overview of a set of genes which may be associated to Cre1 or ACEI. It is the first step towards an understanding of the regulatory roles of the two repressors in cellular pathways, which would be helpful in strain improvement in T. reesei.
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DOI：
10.1007/978-1-61779-310-3_15
发表时间：
2011-01-01
期刊：
NEUROPEPTIDES: METHODS AND PROTOCOLS
影响因子：
--
作者：
Ghorbel, Mohamed T.;Murphy, David
通讯作者：
Murphy, David
影响因子：
2.1
作者：
Takashima, S;Iikura, H;Uozumi, T
通讯作者：
Uozumi, T
DOI：
10.1016/s0167-4781(99)00242-0
发表时间：
2000-02
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
M. A. Noventa-Jordão;Adriana Mendes do Nascimento;M. Goldman;H. Terenzi;G. H. Goldman
通讯作者：
M. A. Noventa-Jordão;Adriana Mendes do Nascimento;M. Goldman;H. Terenzi;G. H. Goldman
影响因子：
4.8
作者：
Saloheimo, A;Aro, N;Penttilä, M
通讯作者：
Penttilä, M
影响因子：
3
作者：
Mogensen, Jesper;Nielsen, H. Bjorn;Nielsen, Jens
通讯作者：
Nielsen, Jens