Genomic and proteomic dissection of the ubiquitous plant pathogen, Armillaria mellea: toward a new infection model system.
Genomic and proteomic dissection of the ubiquitous plant pathogen, Armillaria mellea: toward a new infection model system.
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DOI：
10.1021/pr301131t
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发表时间：
2013-06-07
影响因子：
4.4
通讯作者：
Doyle, Sean
中科院分区：
文献类型：
--
作者：
Collins, Cassandra;Keane, Thomas M.;Turner, Daniel J.;O'Keeffe, Grainne;Fitzpatrick, David A.;Doyle, Sean
关键词：
蜜环菌是一种重要的植物病原菌。然而，没有大规模的“组学”数据，使新的研究，有限的实验模型可用于调查担子菌致病性。在这里，我们揭示了A.蜜环菌基因组全长58.35Mb，包含14473个基因模式，平均长度1575 bp（4.72个内含子/基因）。串联质谱鉴定了921个菌丝体（n = 629个独特的）和分泌（n = 183个独特的）蛋白质。近100种菌丝体蛋白是种特异性的或以前在蛋白质水平上未鉴定的。在菌丝体和培养上清液提取物中检测到许多蛋白质（n = 111）。信号序列的出现是4倍分泌（50.2%）相比，菌丝体蛋白（12%）。分析表明，该菌含有丰富的糖类降解酶、漆酶和木质素过氧化物酶。Mellea蛋白质组，使人联想到担子菌和子囊菌的糖降解砷。我们发现A.在共培养过程中，蜜环菌对白色念珠菌表现出特异性杀伤作用。对这种相互作用的蛋白质组学研究揭示了防御性和潜在攻击性A. Mellea蛋白（n = 30）。总的来说，我们的数据揭示了新的见解的起源，担子菌毒力，我们提出了一个新的模型系统，为进一步研究，旨在破译真菌的致病机制。
Armillaria mellea is a major plant pathogen. Yet, no large-scale “-omics” data are available to enable new studies, and limited experimental models are available to investigate basidiomycete pathogenicity. Here we reveal that the A. mellea genome comprises 58.35 Mb, contains 14473 gene models, of average length 1575 bp (4.72 introns/gene). Tandem mass spectrometry identified 921 mycelial (n = 629 unique) and secreted (n = 183 unique) proteins. Almost 100 mycelial proteins were either species-specific or previously unidentified at the protein level. A number of proteins (n = 111) was detected in both mycelia and culture supernatant extracts. Signal sequence occurrence was 4-fold greater for secreted (50.2%) compared to mycelial (12%) proteins. Analyses revealed a rich reservoir of carbohydrate degrading enzymes, laccases, and lignin peroxidases in the A. mellea proteome, reminiscent of both basidiomycete and ascomycete glycodegradative arsenals. We discovered that A. mellea exhibits a specific killing effect against Candida albicans during coculture. Proteomic investigation of this interaction revealed the unique expression of defensive and potentially offensive A. mellea proteins (n = 30). Overall, our data reveal new insights into the origin of basidiomycete virulence and we present a new model system for further studies aimed at deciphering fungal pathogenic mechanisms.
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