Experimental approaches for gene regulatory network construction: the chick as a model system.

Experimental approaches for gene regulatory network construction: the chick as a model system.
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DOI:
10.1002/dvg.22359
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发表时间:
2013-05
期刊:
影响因子:
1.5
通讯作者:
Stern, Claudio D.
Stern, Claudio D.
中科院分区:
生物学4区
文献类型:
--
作者:
Streit, Andrea;Tambalo, Monica;Chen, Jingchen;Grocott, Timothy;Anwar, Maryam;Sosinsky, Alona;Stern, Claudio D.

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在胚胎发育期间建立身体计划需要许多信号和转录调节因子以精确的时间序列和空间模式协调行动。在过去的几十年里,描述许多发育过程的分子控制的信息爆炸。下一个挑战是将这些信息整合到逻辑“接线图”中,使基因的作用和输出可视化,具有预测能力并指向关键控制节点。在这里,我们提供了一个实验工作流程,如何构建基因调控网络使用鸡作为模型系统。关键词:转录因子,转录组分析,保守调控元件
Setting up the body plan during embryonic development requires the coordinated action of many signals and transcriptional regulators in a precise temporal sequence and spatial pattern. The last decades have seen an explosion of information describing the molecular control of many developmental processes. The next challenge is to integrate this information into logic ‘wiring diagrams’ that visualise gene actions and outputs, have predictive power and point to key control nodes. Here we provide an experimental workflow on how to construct gene regulatory networks using the chick as model system. Keywords: transcription factors, transcriptome analysis, conserved regulatory elements
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