Structural Mass Spectrometry of Membrane Proteins within Their Native Lipid Environments.

Structural Mass Spectrometry of Membrane Proteins within Their Native Lipid Environments.
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膜蛋白在其天然脂质环境中的结构质谱分析。

DOI:
10.1002/chem.201801556
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发表时间:
2018
期刊:
Chemistry (Weinheim an der Bergstrasse, Germany)
影响因子:
--
通讯作者:
Reading E
Reading E
中科院分区:
--
文献类型:
--
作者:
Reading E

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质谱分析已成为了解膜蛋白结构、功能和动力学的重要结构生物学工具。一般来说,膜蛋白的结构质谱分析是在缺乏天然脂质膜和细胞环境的纯化或重构系统上进行的。然而,这些方法在越来越自然的环境中探测膜蛋白的使用和适应性方面已经取得了进展。在这篇概念文章中,重点介绍了结构质谱技术在天然环境中研究膜蛋白的用途和实用性。
Mass spectrometry has emerged as an important structural biology tool for understanding membrane protein structure, function, and dynamics. Generally, structural mass spectrometry of membrane proteins has been performed on purified or reconstituted systems which lack the native lipid membrane and cellular environments. However, there has been progress in the use and adaptations of these methods for probing membrane proteins within increasingly more native contexts. In this Concept article the use and utility of structural mass spectrometry techniques for studying membrane proteins within native environments are highlighted.
用于亲和力分离的内源性蛋白质组件的天然质谱分析的强大工作流程。
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