Mitochondrial activity of human umbilical cord mesenchymal stem cells.
Mitochondrial activity of human umbilical cord mesenchymal stem cells.
复制标题
DOI：
10.4103/bc.bc_15_21
复制
发表时间：
2021-01
影响因子：
1.9
通讯作者：
Borlongan CV
中科院分区：
文献类型：
--
作者：
Cozene BM;Russo E;Anzalone R;Rocca G;Borlongan CV
人脐带间充质干细胞（hUC-MSCs）作为一种潜在的细胞为基础的治疗退行性疾病。它们提供免疫调节和抗炎特性，多能分化潜力，并且收获时没有伦理问题。目前尚不清楚从人脐带的不同区域收集的MSC是否比其他区域产生更有利的效果。从人脐带的不同区域收获三个MSC群：脐带衬里（CL-MSC）、血管周围区域（PV-MSC）和沃顿氏胶质（WJ-MSC）。间充质标志物（CD 90和CD 73）由所有三个细胞群表达。WJ-MSCs、PV-MSCs和CL-MSCs分别表达干细胞标志物（OCT 4）、内皮细胞粘附分子标志物（CD 146）和单核细胞-巨噬细胞标志物（CD 14）。中风表现为氧和葡萄糖剥夺，导致线粒体功能障碍，从而导致细胞死亡。通过线粒体转移靶向恢复中风脑中的线粒体功能可能有效治疗中风。在体外暴露于环境和OGD条件导致CL-MSC数量减少最少后OGD/R暴露，和PV-MSC表现出最大的线粒体活性。所有三个hUC-MSC群体在正常和病理环境中表现出相似的代谢活性和存活率。这些特征表明hUC-MSC作为再生医学中的有效治疗剂的潜力。
Human umbilical cord mesenchymal stem cells (hUC-MSCs) serve as a potential cell-based therapy for degenerative disease. They provide immunomodulatory and anti-inflammatory properties, multipotent differentiation potential and are harvested with no ethical concern. It is unknown whether MSCs collected from different areas of the human umbilical cord elicit more favorable effects than others. Three MSC populations were harvested from various regions of the human umbilical cord: cord lining (CL-MSCs), perivascular region (PV-MSCs), and Wharton's jelly (WJ-MSCs). Mesenchymal markers (CD90 and CD73) were expressed by all three cell populations. Stemness marker (OCT4), endothelial cell adhesion molecular marker (CD146), and monocyte-macrophage marker (CD14) were expressed by WJ-MSCs, PV-MSCs, and CL-MSCs, respectively. Stroke presents with oxygen and glucose deprivation and leads to dysfunctional mitochondria and consequently cell death. Targeting the restoration of mitochondrial function in the stroke brain through mitochondrial transfer may be effective in treating stroke. In vitro exposure to ambient and OGD conditions resulted in CL-MSCs number decreasing the least post-OGD/R exposure, and PV-MSCs exhibiting the greatest mitochondrial activity. All three hUC-MSC populations presented similar metabolic activity and survival in normal and pathologic environments. These characteristics indicate hUC-MSCs potential as a potent therapeutic in regenerative medicine.
登录
查看更多内容
影响因子：
4.8
作者：
Russo, Eleonora;Lee, Jea-Young;Borlongan, Cesar V.
通讯作者：
Borlongan, Cesar V.
影响因子：
6
作者：
Davies JE;Walker JT;Keating A
通讯作者：
Keating A
影响因子：
3.7
作者：
Acosta, Sandra A.;Tajiri, Naoki;Borlongan, Cesar V.
通讯作者：
Borlongan, Cesar V.
影响因子：
4.8
作者：
Lee, Jea-Young;Tuazon, Julian P.;Borlongan, Cesario V.
通讯作者：
Borlongan, Cesario V.
影响因子：
6.1
作者：
Emerich, Dwaine F.;Thanos, Christopher G.;Kordower, Jeffrey H.
通讯作者：
Kordower, Jeffrey H.