3D-Printed COVID-19 immunosensors with electronic readout.
3D-Printed COVID-19 immunosensors with electronic readout.
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DOI：
10.1016/j.cej.2021.131433
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发表时间：
2021-12-01
期刊：
影响因子：
--
通讯作者：
Pumera M
中科院分区：
文献类型：
--
作者：
Muñoz J;Pumera M
3D打印技术通过分散和按需制造不同的个人防护设备和医疗器械，为抗击COVID-19全球大流行事件带来了光明。尽管如此，由于该技术仍处于早期阶段，因此使用3D打印电子设备进行抗原检测开发几乎是一个未开发的领域。本文报道了一种通过表面工程实现的稳健且通用的自下而上的生物功能化方法，旨在为制造第一个具有电子读数的3D打印COVID-19免疫传感器原型提供基础。通过将COVID-19重组蛋白共价锚定在3D打印的石墨烯基纳米复合材料电极表面上，构建了3D打印的COVID-19免疫传感器。电读出依赖于通过竞争性测定与单克隆COVID-19抗体相互作用后阻抗监测电极/电解质界面处的变化，这一事实阻碍了基准氧化还原标记物的氧化还原转化。总的来说，开发的3D打印系统在缓冲和人类血清样品中都表现出有前途的电分析能力，显示出优异的线性响应，检测限为痕量水平（0.5 ± 0.1 μg·mL−1）。这些成就证明了3D打印设备的光速分布的优势，以及本地化的护理点低成本打印和生物电子设备，以帮助遏制新出现的传染病（如COVID-19）的传播。该技术适用于任何COVID-19后的SARS疾病。
3D printing technology has brought light in the fight against the COVID-19 global pandemic event through the decentralized and on-demand manufacture of different personal protective equipment and medical devices. Nonetheless, since this technology is still in an early stage, the use of 3D-printed electronic devices for antigen test developments is almost an unexplored field. Herein, a robust and general bottom-up biofunctionalization approach via surface engineering is reported aiming at providing the bases for the fabrication of the first 3D-printed COVID-19 immunosensor prototype with electronic readout. The 3D-printed COVID-19 immunosensor was constructed by covalently anchoring the COVID-19 recombinant protein on a 3D-printed graphene-based nanocomposite electrode surface. The electrical readout relies on impedimetrically monitoring changes at the electrode/electrolyte interface after interacting with the monoclonal COVID-19 antibody via competitive assay, fact that hinders the redox conversion of a benchmark redox marker. Overall, the developed 3D-printed system exhibits promising electroanalytical capabilities in both buffered and human serum samples, displaying an excellent linear response with a detection limit at trace levels (0.5 ± 0.1 μg·mL−1). Such achievements demonstrate advantage of light-of-speed distribution of 3D printing datafiles with localized point-of-care low-cost printing and bioelectronic devices to help contain the spread of emerging infectious diseases such as COVID-19. This technology is applicable to any post-COVID-19 SARS diseases.
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影响因子：
4.3
作者：
Ghosh, Kalyan;Ng, Siowwoon;Pumera, Martin
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19
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影响因子：
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作者：
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通讯作者：
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影响因子：
8.4
作者：
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通讯作者：
Bonacin, Juliano A.
DOI：
10.1016/j.trac.2020.116081
发表时间：
2020-12
期刊：
Trends in analytical chemistry : TRAC
影响因子：
--
作者：
Lim RRX;Bonanni A
通讯作者：
Bonanni A