Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis.
Control of Oriented Tissue Growth through Repression of Organ Boundary Genes Promotes Stem Morphogenesis.
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DOI：
10.1016/j.devcel.2016.08.013
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发表时间：
2016-10-24
影响因子：
11.8
通讯作者：
Sablowski, Robert
中科院分区：
文献类型：
--
作者：
Bencivenga, Stefano;Serrano-Mislata, Antonio;Bush, Max;Fox, Samantha;Sablowski, Robert
茎的起源是植物发育的一个重要但鲜为人知的方面，部分原因是茎起源于茎尖分生组织中一个相对难以接近的区域，称为肋区（RZ）。我们开发了定量三维图像分析和克隆分析工具，揭示了拟南芥同源结构域蛋白REPLUMLESS （RPL）在RZ的中心和外周区域建立了不同的定向细胞分裂和生长模式。对目标基因的全基因组筛选将RPL直接与许多关键的茎发育途径联系起来，包括器官边界的发育；因此，在rpl突变体中，器官边界基因光敏HYPOCOTYL 4的突变恢复了RZ功能和茎的生长。我们的工作为研究对作物改良具有重要意义的发育过程开辟了道路，并强调了3D器官生长中明显的简单变化如何反映定向细胞活动中更复杂的内部变化。RPL与调控茎部器官发生的许多关键基因相互作用。RPL通过直接抑制器官边界基因来控制定向生长。茎的起源是我们对植物发育认识的一个主要缺口。Bencivenga等研究表明，拟南芥在茎形成过程中，调控基因REPLUMLESS通过抑制器官边界基因控制细胞分裂和生长的三维模式。
The origin of the stem is a major but poorly understood aspect of plant development, partly because the stem initiates in a relatively inaccessible region of the shoot apical meristem called the rib zone (RZ). We developed quantitative 3D image analysis and clonal analysis tools, which revealed that the Arabidopsis homeodomain protein REPLUMLESS (RPL) establishes distinct patterns of oriented cell division and growth in the central and peripheral regions of the RZ. A genome-wide screen for target genes connected RPL directly to many of the key shoot development pathways, including the development of organ boundaries; accordingly, mutation of the organ boundary gene LIGHT-SENSITIVE HYPOCOTYL 4 restored RZ function and stem growth in the rpl mutant. Our work opens the way to study a developmental process of importance to crop improvement and highlights how apparently simple changes in 3D organ growth can reflect more complex internal changes in oriented cell activities. Image and sector analysis revealed 3D growth patterns in early stem development Arabidopsis RPL controls oriented cell division and growth in the rib meristem RPL interacts with many of the key genes that regulate shoot organogenesis RPL controls oriented growth by directly repressing organ boundary genes The origin of the stem is a major gap in our understanding of plant development. Bencivenga et al. show that during stem initiation in Arabidopsis, the regulatory gene REPLUMLESS controls three-dimensional patterns of cell division and growth through repression of organ boundary genes.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
9.8
作者：
Boehm B;Westerberg H;Lesnicar-Pucko G;Raja S;Rautschka M;Cotterell J;Swoger J;Sharpe J
通讯作者：
Sharpe J
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
DOI：
10.1073/pnas.1011866108
发表时间：
2011-04-12
影响因子：
11.1
作者：
Besson, Sebastien;Dumais, Jacques
通讯作者：
Dumais, Jacques
影响因子：
7.4
作者：
Khan, Madiha;Xu, Mingli;Hepworth, Shelley R.
通讯作者：
Hepworth, Shelley R.