Engineered Faceted Cerium Oxide Nanoparticles for Therapeutic miRNA Delivery.
Engineered Faceted Cerium Oxide Nanoparticles for Therapeutic miRNA Delivery.
复制标题
DOI：
10.3390/nano12244389
复制
发表时间：
2022-12-09
期刊：
影响因子：
--
通讯作者：
Seal S
中科院分区：
文献类型：
--
作者：
Fu Y;Kolanthai E;Neal CJ;Kumar U;Zgheib C;Liechty KW;Seal S
一般来说，伤口愈合是一个高度有序的过程，具有不同的炎症、增殖和重塑阶段。然而，在糖尿病患者中，这些阶段的进展常常受到氧化应激水平升高和持续炎症的阻碍。我们之前的研究表明，氧化铈纳米颗粒 (CNP) 与治疗性 microRNA146a (miR146a) 结合可以通过靶向 NFκB 通路、减少氧化应激和炎症来有效促进伤口愈合。在本研究中，我们考虑了纳米材料表面刻面和形态对 miRNA 递送功效的潜在影响。与八面体-CNPs和立方-CNPs相比，棒-CNPs表现出更高的负载能力。此外，在比较颗粒形态对伤口愈合功效的影响时，评估了几种生物活性标志物，并将其归因于基因递送和活性氧（ROS）清除特性的综合影响。在细胞治疗研究中，rod-CNP-miR146a 显示出最大的 miR146a 递送到细胞中的能力。然而，仅在八面体-CNP-miR146a 中观察到 IL-6 的减少，这表明 miRNA 递送的功效是多种因素综合作用的结果。总的来说，我们的结果对具有不同形态的 CNP 的相对递送效率提高了 miRNA 递送效率提供了启示。
In general, wound healing is a highly ordered process, with distinct phases of inflammation, proliferation, and remodeling. However, among diabetic patients, the progression through these phases is often impeded by increased level of oxidative stress and persistent inflammation. Our previous studies demonstrated that cerium oxide nanoparticles (CNPs) conjugated with therapeutic microRNA146a (miR146a) could effectively enhance wound healing by targeting the NFκB pathway, reducing oxidative stress and inflammation. In the present study, we consider the potential effects of nanomaterial surface-faceting and morphology on the efficacy of miRNA delivery. Compared with octahedral-CNPs and cubic-CNPs, rod-CNPs exhibited higher loading capacity. In addition, in comparing the influence of particle morphology on wound healing efficacy, several markers for bioactivity were evaluated and ascribed to the combined effects of the gene delivery and reactive oxygen species (ROS) scavenging properties. In the cellular treatment study, rod-CNP-miR146a displayed the greatest miR146a delivery into cells. However, the reduction of IL-6 was only observed in the octahedral-CNP-miR146a, suggesting that the efficacy of the miRNA delivery is a result of the combination of various factors. Overall, our results give enlightenments into the relative delivery efficiency of the CNPs with different morphology enhancing miRNA delivery efficacy.
登录
查看更多内容
影响因子：
4.4
作者：
Nolan, Michael
通讯作者：
Nolan, Michael
影响因子：
7.3
作者：
Dhall, A.;Burns, A.;Self, W.
通讯作者：
Self, W.
影响因子：
14
作者：
Heckert, Eric G.;Karakoti, Ajay S.;Self, William T.
通讯作者：
Self, William T.
二氧化铈纳米颗粒获得的超氧化物清除能力
影响因子：
16.6
作者：
Li, Yuanyuan;He, Xiao;Zhang, Zhiyong
通讯作者：
Zhang, Zhiyong
影响因子：
8.6
作者：
Ganduglia-Pirovano, M. Veronica;Da Silva, Juarez L. F.;Sauer, Joachim
通讯作者：
Sauer, Joachim