Mammalian myosin Iα is concentrated near the plasma membrane in nerve growth cones
Mammalian myosin Iα is concentrated near the plasma membrane in nerve growth cones
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哺乳动物肌球蛋白 Iα 集中在神经生长锥质膜附近
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
P. Bridgman
中科院分区：
文献类型：
--
作者：
A. K. Lewis;P. Bridgman
为了确定非常规肌球蛋白在神经生长中是否起作用，用大鼠脑源性哺乳动物肌球蛋白Iα(MMIα)抗体标记培养的大鼠颈上神经节神经细胞。使用旨在提高准确确定抗体标记和细胞结构之间的关系以便绘制这种肌球蛋白的分布和结构关联的能力的制备程序，在光学和电子显微镜分辨率水平上进行观察。免疫荧光显示Mmiα在神经细胞体、神经突起和生长锥体中呈点状分布。在生长锥体中，MMIα染色有时在前缘肌动蛋白含量较高的薄外围区域升高。用胶体金标记抗体的免疫电子显微镜显示，在生长锥体中，Mmiα不存在于膜细胞器中，主要集中在靠近细胞膜的细胞皮质。皮质标记均匀地分布在上膜和下膜之间。在肌动蛋白细胞骨架受到干扰或被移除的情况下，MMIα标记的质膜结合持续存在，这表明MMIα的一部分与质膜之间存在直接联系。MMIα标记也与非皮质肌动蛋白细胞骨架有关。使用细胞松弛素B部分破坏肌动蛋白细胞骨架只会导致MMIα标记的子集重新分布。这些数据表明，MMIα、肌动蛋白细胞骨架和生长锥体中的质膜之间存在复杂的关系。与其在生长锥体中的定位相反，在神经元细胞体中，Mmiα也与管室状结构有关。这表明，在这个位置，Mmiα可能作为细胞器马达或被被动地运输到囊泡的质膜上。
To determine if unconventional myosins play a role in nerve outgrowth, antibodies specific for rat brain derived mammalian myosin I alpha (MMI alpha) were used to label cultured rat superior cervical ganglion nerve cells. Observations were made at both the light and electron microscopic level of resolution using preparative procedures designed to enhance the ability to precisely determine the relationship between antibody label and cellular structures in order to map the distribution and structural association of this myosin. Immunofluorescence showed that MMI alpha has a punctate distribution throughout the nerve cell body, neurites, and growth cones. In growth cones, MMI alpha staining is sometimes elevated in thin peripheral regions of high actin content at the leading edge. Immunoelectron microscopy using colloidal gold conjugated antibodies showed that in growth cones MMI alpha is absent from membranous organelles and is concentrated primarily in the cell cortex adjacent to the cell membrane. The cortical label is equally distributed between upper and lower membranes. The plasma membrane association of the MMI alpha label persists under conditions in which the actin cytoskeleton is perturbed or removed, suggesting a direct association between a fraction of MMI alpha and the plasma membrane. MMI alpha label is also associated with the non-cortical actin cytoskeleton. Partial disruption of the actin cytoskeleton using cytochalasin B causes redistribution of only a subset of MMI alpha label. These data suggest a complex relationship between MMI alpha, the actin cytoskeleton, and the plasma membrane in the growth cone. In contrast to its localization in the growth cone, in neuronal cell bodies MMI alpha is also associated with tubulovesicular structures. This suggests that at this location MMI alpha may either act as an organelle motor or is passively transported to the plasma membrane on vesicles.
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DOI：
10.1073/pnas.90.2.659
发表时间：
1993-01-15
影响因子：
11.1
作者：
GOODSON, HV;SPUDICH, JA
通讯作者：
SPUDICH, JA
影响因子：
--
作者：
CHENEY, RE;RILEY, MA;MOOSEKER, MS
通讯作者：
MOOSEKER, MS
影响因子：
--
作者：
GOODSON, HV;SPUDICH, JA
通讯作者：
SPUDICH, JA
新型 130 kDa 大鼠肝脏肌球蛋白-1 将使肌动蛋白丝易位。
影响因子：
--
作者：
Williams,R;Coluccio,LM
通讯作者：
Coluccio,LM
哺乳动物肝脏中的肌球蛋白-I。
影响因子：
--
作者：
Coluccio,LM;Conaty,C
通讯作者：
Conaty,C