MicroRNA miR171b Positively Regulates Resistance to Huanglongbing of Citrus.
MicroRNA miR171b Positively Regulates Resistance to Huanglongbing of Citrus.
复制标题
DOI：
10.3390/ijms24065737
复制
发表时间：
2023-03-17
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
黄龙病是世界上危害最严重的柑橘病害之一，造成了巨大的经济损失。然而，尚未开发出保护柑橘免受HLB影响的有效方法。microRNA（miRNA）介导的基因表达调控是控制植物病害的有用工具，但参与调节HLB抗性的miRNA尚未被鉴定。在这项研究中，我们发现miR 171 b正调控柑橘对HLB的抗性。在用HLB细菌感染后，在对照植物中的第二个月检测到细菌。然而，在miR 171 b过表达的转基因柑橘植株中，直到第24个月才能检测到细菌。RNA-seq数据表明，多种途径，如光合作用，植物-病原体相互作用，MAPK信号通路等，与对照相比，miR 171 b过表达植株对HLB的抗性可能与提高有关。最后，我们确定miR 171 b可以靶向SCARECROW样（SCL）基因下调其表达，从而导致对HLB胁迫的抗性增强。总之，我们的研究结果表明，miR 171 b在柑橘HLB抗性中起着积极的调节作用，并提供了一个新的见解，在柑橘适应HLB胁迫的miRNAs的作用。
Huanglongbing (HLB) is one of the most severe citrus diseases in the world, causing huge economic losses. However, efficient methods of protecting citrus from HLB have not yet been developed. microRNA (miRNA)-mediated regulation of gene expression is a useful tool to control plant diseases, but the miRNAs involved in regulating resistance to HLB have not yet been identified. In this study, we found that miR171b positively regulated resistance to HLB in citrus. Upon infection with HLB bacteria, the bacteria were detected in the second month in the control plants. However, in the miR171b-overexpressing transgenic citrus plants, the bacteria could not be detected until the 24th month. RNA-seq data indicated that multiple pathways, such as photosynthesis, plant–pathogen interaction, the MAPK signaling pathway, etc., might be involved in improving the resistance to HLB in miR171b-overexpressing plants compared with the control. Finally, we determined that miR171b could target SCARECROW-like (SCL) genes to downregulate their expression, which then led to promoted resistance to HLB stress. Collectively, our results demonstrate that miR171b plays a positive regulatory role in resistance to citrus HLB, and provides a new insight into the role of miRNAs in the adaptation of citrus to HLB stress.
影响因子：
5.6
作者：
Yang, Zhengmei;Dong, Tingting;Dai, Xibin;Wei, Yiliang;Fang, Yujie;Zhang, Lei;Zhu, Mingku;Nawaz, Ghazala;Cao, Qinghe;Xu, Tao
通讯作者：
Xu, Tao