Comparative Analysis of Salt Responsive MicroRNAs in Two Sweetpotato [Ipomoea batatas (L.) Lam.] Cultivars With Different Salt Stress Resistance.
Comparative Analysis of Salt Responsive MicroRNAs in Two Sweetpotato [Ipomoea batatas (L.) Lam.] Cultivars With Different Salt Stress Resistance.
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两种甘薯盐敏感microRNA的比较分析Lam.]耐盐性不同的品种。
DOI：
10.3389/fpls.2022.879819
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发表时间：
2022
影响因子：
5.6
通讯作者：
Xu, Tao
中科院分区：
文献类型：
--
作者：
Yang, Zhengmei;Dong, Tingting;Dai, Xibin;Wei, Yiliang;Fang, Yujie;Zhang, Lei;Zhu, Mingku;Nawaz, Ghazala;Cao, Qinghe;Xu, Tao
甘薯（L.）林。[]是一种重要的粮食、蔬菜和经济作物，但其产量受土壤盐分的影响显著。mirna是一类内源性非编码小rna，在植物抗盐胁迫中起重要作用。然而，在盐胁迫下的甘薯中，mirna的功能在很大程度上仍然未知。之前，我们在一个盐敏感的甘薯品种“徐蜀32”中鉴定了盐敏感的mirna。在本研究中，我们通过高通量深度测序鉴定了另一个耐盐品种“徐树22”的miRNAs，并比较了这两个盐敏感性不同的品种的盐响应miRNAs。我们在“徐舒22”中鉴定了687种mirna，其中包括514种已知mirna和173种新mirna。其中，在“徐树32”和“徐树22”中特异表达的有72个，共表达的有109个。“徐树32”和“徐树22”基因型比较发现，在盐胁迫下，“徐树32”和“徐树22”基因型共有235个表达差异明显的mirna， 177个盐响应mirna在盐胁迫下表达差异明显。使用target Finder工具和降解组测序预测和鉴定mirna的靶基因。结果表明，大多数靶点是与代谢和应激反应相关的转录因子和蛋白质。基因本体分析表明，这些靶基因参与了盐胁迫反应和次生氧化还原代谢的关键通路。对不同盐敏感性甘薯品种的盐响应miRNA进行比较分析，有助于了解不同基因型甘薯中miRNA的调控模式，有助于今后通过miRNA操作改善甘薯的农艺性状。
Sweetpotato [Ipomoea batatas (L.) Lam.] is an important food, vegetable and economic crop, but its productivity is remarkably affected by soil salinity. MiRNAs are a class of endogenous non-coding small RNAs that play an important role in plant resistance to salt stress. However, the function of miRNAs still remains largely unknown in sweetpotato under salt stress. Previously, we identified salt-responsive miRNAs in one salt-sensitive sweetpotato cultivar “Xushu 32.” In this study, we identified miRNAs in another salt-tolerant cultivar “Xushu 22” by high-throughput deep sequencing and compared the salt-responsive miRNAs between these two cultivars with different salt sensitivity. We identified 687 miRNAs in “Xushu 22,” including 514 known miRNAs and 173 novel miRNAs. Among the 759 miRNAs from the two cultivars, 72 and 109 miRNAs were specifically expressed in “Xushu 32” and “Xushu 22,” respectively, and 578 miRNAs were co-expressed. The comparison of “Xushu 32” and “Xushu 22” genotypes showed a total of 235 miRNAs with obvious differential expression and 177 salt-responsive miRNAs that were obviously differently expressed between “Xushu 32” and “Xushu 22” under salt stress. The target genes of the miRNAs were predicted and identified using the Target Finder tool and degradome sequencing. The results showed that most of the targets were transcription factors and proteins related to metabolism and stress response. Gene Ontology analysis revealed that these target genes are involved in key pathways related to salt stress response and secondary redox metabolism. The comparative analysis of salt-responsive miRNAs in sweetpotato cultivars with different salt sensitivity is helpful for understanding the regulatory pattern of miRNA in different sweetpotato genotypes and improving the agronomic traits of sweetpotato by miRNA manipulation in the future.
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影响因子：
7.4
作者：
Guo, Qi;Liu, Lei;Rupasinghe, Thusitha W. T.;Roessner, Ute;Barkla, Bronwyn J.
通讯作者：
Barkla, Bronwyn J.
影响因子：
3.7
作者：
Leyva-González MA;Ibarra-Laclette E;Cruz-Ramírez A;Herrera-Estrella L
通讯作者：
Herrera-Estrella L
影响因子：
9.2
作者：
Addo-Quaye, Charles;Eshoo, Tifani W.;Axtell, Michael J.
通讯作者：
Axtell, Michael J.
影响因子：
4.3
作者：
Li, Yuxia;Zhang, Lei;Xu, Tao
通讯作者：
Xu, Tao
影响因子：
5.8
作者：
Addo-Quaye, Charles;Miller, Webb;Axtell, Michael J.
通讯作者：
Axtell, Michael J.