Real-time imaging of head and neck squamous cell carcinomas using confocal micro-endoscopy and applicable dye: A preliminary study.
Real-time imaging of head and neck squamous cell carcinomas using confocal micro-endoscopy and applicable dye: A preliminary study.
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使用共焦显微内窥镜和适用染料对头颈鳞状细胞癌进行实时成像：初步研究。
DOI：
10.1016/j.anl.2020.02.001
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Mizoguchi A.
中科院分区：
文献类型：
--
作者：
Shinohara S;Funabiki K;Kikuchi M;Takebayashi S;Hamaguchi K;Hara S;Yamashita D;Imai Y;Mizoguchi A.
目的共聚焦激光内镜（CLE）是一种能够实时观察病变的技术（光学活检），已成功应用于胃肠病学的临床。最近，它也被引入头颈部鳞状细胞癌（HNSCC）的诊断。我们之前设计了一个自制CLE，它可以提供双铬色图像，局部对比剂对患者是安全的。在此，我们报告了一项试点研究的结果，使用我们自制的CLE对正常和癌组织进行成像。本研究旨在比较HNSCC与正常粘膜的特征，并建立HNSCC的活体实时光学活检方法。方法选取10例行手术切除的非鳞状细胞癌组织。解剖标本首先在473 nm激光激发和进一步的光学观察下评估其自荧光光谱分布。在获取图像的同时，用光谱仪实时测量了反射荧光信号的自荧光光谱和强度。随后，在473 nm激光激发下，将吖啶黄碱涂于标本上，荧光标记细胞核，观察正常组织与癌组织的差异。最后，在473 nm和561 nm激光激发下，用吖黄碱和食用红No.106双染色观察正常组织和癌组织的细胞核和细胞质。结果在473 nm激光激发下，肿瘤组织的自荧光图像信号明显低于正常组织。应用吖啶黄碱后，癌粘膜与正常粘膜在核大小和形状的均匀性上有明显差异。在正常粘膜中，细胞排列有序，每个细胞类似于青蛙的卵。相反，在癌组织中，细胞密度更高，细胞排列不那么有序。同时使用吖啶黄素和食品红No.106，图像变得更加生动，但更复杂，因为细胞质的红色染料染色在不同波长出现荧光。结论基于CLE和条件的实时活体成像可用于区分癌组织和正常粘膜，而无需侵入性活检。
ObjectivesConfocal laser endomicroscopy (CLE) is a technology that enables microscopic visualization of lesions in real-time (optical biopsy) and has been successfully applied for clinical use in gastroenterology. Recently, it was also introduced for head and neck squamous cell carcinoma (HNSCC) diagnostics. We previously designed a self-made CLE, which can provide bichrome images, with topical contrast agents that are safe for use in patients. Herein, we report findings of a pilot study using our self-made CLE to image pairs of normal and cancerous tissues. This study aimed to characterize the features of HNSCC compared with normal mucosa and to establish a methodology of in vivo real-time optical biopsy of HNSCCs.MethodsHNSCC tissues were acquired from 10 patients who underwent surgical resection. Dissected specimens were first evaluated for their auto-fluorescence spectral profiles with 473 nm laser excitation and further optical observation. While obtaining the image, auto-fluorescence spectrum and intensity of the reflectance fluorescent signals were measured in real-time by a spectrometer. Subsequently, acriflavine was applied to the specimen to fluorescently label the nuclei and observe the difference between normal and cancerous tissues with 473 nm laser excitation. Finally, double staining with acriflavine and edible Food Red No.106 was performed to observe both nuclei and the cytoplasm of normal and cancerous tissues at 473 nm and 561 nm laser excitation.ResultsLower signals were detected from auto-fluorescence images of cancer tissues than normal tissues with 473 nm laser excitation. After acriflavine application, there was a clear difference between cancer and normal mucosa in the uniformity of nuclear size and shape. In normal mucosa, cells were arranged in an orderly manner, with each cell resembling a frog's egg. By contrast, in cancer tissues, the cell density was higher, and the cellular arrangement was less orderly. Using both acriflavine and Food Red No.106, images became more vivid, but more complicated because red dye staining of the cytoplasm emerged as fluorescence at different wavelengths.ConclusionsReal-time in vivo imaging using the newly developed CLE and conditions may be used to distinguish cancer tissue from normal mucosa without invasive biopsy.
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DOI：
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发表时间：
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DOI：
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发表时间：
1999-10
期刊：
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