Differential gene expression in proximal and distal nerve segments of rats with sciatic nerve injury during Wallerian degeneration.
Differential gene expression in proximal and distal nerve segments of rats with sciatic nerve injury during Wallerian degeneration.
复制标题
华勒变性坐骨神经损伤大鼠近远端神经节段差异基因表达
DOI：
10.4103/1673-5374.135325
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发表时间：
2014-06-15
影响因子：
6.1
通讯作者：
Yao D
中科院分区：
文献类型：
--
作者：
Jiang N;Li H;Sun Y;Yin D;Zhao Q;Cui S;Yao D
沃勒变性是神经科学中的一个主要研究课题。在Wallerian变性的不同阶段，大量基因受到差异调节：转录因子激活，免疫应答，髓鞘细胞分化和去分化。虽然周围神经损伤后坐骨神经远段的基因表达反应是已知的，但近段和远段之间的基因表达差异仍不清楚。在本研究中，在大鼠中，我们使用微阵列来分析基因表达，生物过程和信号通路的变化在近端和远端段的坐骨神经进行沃勒变性。结果表明，伤后7-14 d，共检测到6,000多个基因的差异表达，并鉴定出20种表达趋势，主要分布在近端和远端节段。差异表达的基因涉及细胞分化、胞质分裂、神经元分化、神经发育和轴突再生。此外，11个生物学过程，涉及刺激反应，细胞凋亡，炎症反应，免疫反应，信号转导，蛋白激酶活性和细胞增殖。使用实时定量PCR，蛋白质印迹分析和免疫组化，芯片数据进行验证的四个基因：水通道蛋白-4，白细胞介素1受体样1，基质金属蛋白酶-12和periaxin。我们的研究确定了沃勒变性过程中神经近端和远端段的差异基因表达，分析了这些基因的动态生物学变化，并为详细研究沃勒变性过程中神经损伤和修复提供了一个有用的平台。
Wallerian degeneration is a subject of major interest in neuroscience. A large number of genes are differentially regulated during the distinct stages of Wallerian degeneration: transcription factor activation, immune response, myelin cell differentiation and dedifferentiation. Although gene expression responses in the distal segment of the sciatic nerve after peripheral nerve injury are known, differences in gene expression between the proximal and distal segments remain unclear. In the present study in rats, we used microarrays to analyze changes in gene expression, biological processes and signaling pathways in the proximal and distal segments of sciatic nerves undergoing Wallerian degeneration. More than 6,000 genes were differentially expressed and 20 types of expression tendencies were identified, mainly between proximal and distal segments at 7–14 days after injury. The differentially expressed genes were those involved in cell differentiation, cytokinesis, neuron differentiation, nerve development and axon regeneration. Furthermore, 11 biological processes were represented, related to responses to stimuli, cell apoptosis, inflammatory response, immune response, signal transduction, protein kinase activity, and cell proliferation. Using real-time quantitative PCR, western blot analysis and immunohistochemistry, microarray data were verified for four genes: aquaporin-4, interleukin 1 receptor-like 1, matrix metalloproteinase-12 and periaxin. Our study identifies differential gene expression in the proximal and distal segments of a nerve during Wallerian degeneration, analyzes dynamic biological changes of these genes, and provides a useful platform for the detailed study of nerve injury and repair during Wallerian degeneration.
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影响因子：
5.4
作者：
Gao, Bo;Sun, Wang;Xue, Dongbo
通讯作者：
Xue, Dongbo
影响因子：
5.6
作者：
Yao, Dengbing;Li, Meiyuan;Gu, Xiaosong
通讯作者：
Gu, Xiaosong
影响因子：
14.9
作者：
Huang DW;Sherman BT;Tan Q;Kir J;Liu D;Bryant D;Guo Y;Stephens R;Baseler MW;Lane HC;Lempicki RA
通讯作者：
Lempicki RA
影响因子：
5.3
作者：
Stepanek, L;Stoker, AW;Bixby, JL
通讯作者：
Bixby, JL
DOI：
10.1073/pnas.1001948107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Vargas, Mauricio E.;Watanabe, Junryo;Barres, Ben A.
通讯作者：
Barres, Ben A.