A survey of 6,300 genomic fragments for cis-regulatory activity in the imaginal discs of Drosophila melanogaster.
A survey of 6,300 genomic fragments for cis-regulatory activity in the imaginal discs of Drosophila melanogaster.
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DOI：
10.1016/j.celrep.2012.09.010
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发表时间：
2012-10-25
期刊：
影响因子：
8.8
通讯作者：
Mann RS
中科院分区：
文献类型：
--
作者：
Jory A;Estella C;Giorgianni MW;Slattery M;Laverty TR;Rubin GM;Mann RS
分析了来自果蝇基因组的6，334个片段在3龄幼虫成虫盘中驱动GAL 4报告基因表达的能力。1，194个报告基因驱动眼睛、触角、腿、翅膀、背鳍或生殖器成虫盘中的表达。这些模式从大区域到单个细胞不等。大约75%的活性片段驱动多个盘中的表达; 20%在腹侧而非背侧盘（腿、生殖器和触角）中表达，而~23%在背侧而非腹侧盘（翅膀、背索和眼睛）中表达。几种模式，例如在腿部弦音器官中，出现了令人惊讶的大量次数。对DNA序列基序的无偏搜索表明候选转录因子可能调节具有共享活性的增强子。总之，这些表达模式提供了一个宝贵的资源，社会和提供一个广泛的概述转录调控信息是如何分布在果蝇基因组。
6,334 fragments from the genome of Drosophila melanogaster were analyzed for their ability to drive expression of GAL4 reporter genes in the 3rd instar larval imaginal discs. 1,194 reporter genes drove expression in the eye, antenna, leg, wing, haltere, or genital imaginal discs. The patterns ranged from large regions to individual cells. About 75% of the active fragments drove expression in multiple discs; 20% were expressed in ventral, but not dorsal, discs (legs, genital, and antenna), while ~23% were expressed in dorsal, but not ventral discs (wing, haltere, and eye). Several patterns, for example within the leg chordotonal organ, appeared a surprisingly large number of times. Unbiased searches for DNA sequence motifs suggest candidate transcription factors that may regulate enhancers with shared activities. Together, these expression patterns provide a valuable resource to the community and offer a broad overview of how transcriptional regulatory information is distributed in the Drosophila genome.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
4.5
作者：
Estella C;Mann RS
通讯作者：
Mann RS
影响因子：
4
作者：
Barolo, Scott
通讯作者：
Barolo, Scott
影响因子：
14.9
作者：
Gallo SM;Gerrard DT;Miner D;Simich M;Des Soye B;Bergman CM;Halfon MS
通讯作者：
Halfon MS
影响因子：
9.2
作者：
Perry, Michael W.;Boettiger, Alistair N.;Bothma, Jacques P.;Levine, Michael
通讯作者：
Levine, Michael