The vesicular trafficking system component MIN7 is required for minimizing Fusarium graminearum infection
The vesicular trafficking system component MIN7 is required for minimizing Fusarium graminearum infection
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最小化禾谷镰刀菌感染需要囊泡运输系统组件 MIN7
DOI：
10.1101/2020.03.16.994095
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Machado Wood A
中科院分区：
文献类型：
--
作者：
Machado Wood A
植物已经发展出复杂的防御机制，称为先天免疫，以保护自己免受各种病原体的侵害。植物通常通过在膜囊泡中合成并将各种抗微生物化合物、蛋白质和小RNA递送到原发感染部位来快速响应病原体攻击。关于植物-病原体相互作用中囊泡运输的重要性的许多证据来自涉及模式植物的研究，而这一过程在作物植物中的研究相对不足。在这里，我们评估了先前在拟南芥中与免疫有关的囊泡运输系统组分是否在与禾谷镰刀菌的相互作用中发挥作用，禾谷镰刀菌是一种以其引起小麦赤霉病的能力而闻名的真菌病原体。在所分析的囊泡运输突变体中，AtMin 7基因中的两个独立的T-DNA插入突变体显示出对F的敏感性显著增强。禾谷早熟禾早期的研究确定了这个基因，编码一个ARF-GEF蛋白，作为细菌病原体番茄假单胞菌的HopM 1效应子的靶标，它使MIN 7不稳定，导致其降解和削弱宿主防御。为了测试这种关键的囊泡运输组分是否也有助于作物的防御，我们在小麦中鉴定了候选TaMin 7基因，并通过病毒诱导的基因沉默敲低了它们的表达。TaMin 7基因被沉默的小麦植株表现出显著更多的赤霉病。这表明模型和作物植物中MIN 7功能的破坏损害了先天免疫信号或产物的运输，导致对各种病原体的过敏性。
Plants have developed intricate defense mechanisms, referred to as innate immunity, to defend themselves against a wide range of pathogens. Plants often respond rapidly to pathogen attack by the synthesis and delivery to the primary infection sites of various antimicrobial compounds, proteins, and small RNA in membrane vesicles. Much of the evidence regarding the importance of vesicular trafficking in plant–pathogen interactions comes from studies involving model plants whereas this process is relatively understudied in crop plants. Here we assessed whether the vesicular trafficking system components previously implicated in immunity in Arabidopsis play a role in the interaction withFusarium graminearum, a fungal pathogen well-known for its ability to causeFusariumhead blight disease in wheat. Among the analysed vesicular trafficking mutants, two independent T-DNA insertion mutants in theAtMin7gene displayed a markedly enhanced susceptibility toF. graminearum. Earlier studies identified this gene, encoding an ARF-GEF protein, as a target for the HopM1 effector of the bacterial pathogenPseudomonas syringaepv.tomato, which destabilizes MIN7 leading to its degradation and weakening host defenses. To test whether this key vesicular trafficking component may also contribute to defense in crop plants, we identified the candidateTaMin7genes in wheat and knocked-down their expression through virus-induced gene silencing. Wheat plants in whichTaMin7genes were silenced displayed significantly more Fusarium head blight disease. This suggests that disruption of MIN7 function in both model and crop plants compromises the trafficking of innate immunity signals or products resulting in hypersusceptibility to various pathogens.
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