Clostridium frigidicarnis sp. nov., a psychrotolerant bacterium associated with 'blown pack' spoilage of vacuum-packed meats.
Clostridium frigidicarnis sp. nov., a psychrotolerant bacterium associated with 'blown pack' spoilage of vacuum-packed meats.
复制标题
寒冷梭菌 sp.
DOI：
10.1099/00207713-49-4-1539
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
D. Musgrave
中科院分区：
文献类型：
--
作者：
D. Broda;P A Lawson;R. Bell;D. Musgrave
使用多相方法对从真空包装的高温牛肉中分离出的两种耐冷梭状芽胞杆菌菌株进行了表征。该菌株是革兰氏阳性运动杆，在早期静止生长期产生椭圆形近末端孢子。这些菌株具有耐冷性。 pH 值为 7.0 时，它们在 3.8 至 40.5 摄氏度之间生长；它们的最适生长温度为30.0-38.5℃。30℃时，生长的pH范围为4.7至9.5；最适生长pH为6.4-7.2。该生物体具有蛋白水解性和糖分解性、卵磷脂酶阳性和水解明胶。在蛋白胨/酵母提取物/葡萄糖/淀粉肉汤中形成的发酵产物是乙酸盐、乙醇、丁酸盐、异戊酸盐、丁醇、异丁酸盐、草乙酸盐、乳酸盐、氢气和二氧化碳。两个肉品系的DNA G+C组成分别为27.3和28.4 mol%。系统发育分析表明，这些菌株属于梭状芽胞杆菌属的Cluster I（sensu Collins et al., 1994）。这些新菌株在细胞脂肪酸组成、可溶性蛋白质谱和表型特性方面与系统发育相关的梭菌不同。根据表型和基因型特征数据，这些菌株被归入一个新物种，建议将其命名为冷梭菌 (Clostridium frigidicarnis)；菌株 SPL77AT (= DSM 12271T) 是模式菌株。
Two strains of a psychrotolerant Clostridium, isolated from vacuum-packed, temperature-abused beef, were characterized using a multiphasic approach. The strains were Gram-positive motile rods producing elliptical subterminal spores during early stationary growth phase. The strains were psychrotolerant. At pH 7.0, they grew between 3.8 and 40.5 degrees C; their optimum growth temperature was 30.0-38.5 degrees C. At 30 degrees C, the pH range for growth was between 4.7 and 9.5; the optimum pH for growth was 6.4-7.2. The organisms were proteolytic and saccharolytic, lecithinase-positive and hydrolysed gelatin. The fermentation products formed in peptone/yeast extract/glucose/starch broth were acetate, ethanol, butyrate, isovalerate, butanol, isobutyrate, oxalacetate, lactate, hydrogen and carbon dioxide. The DNA G + C compositions of the two meat strains were 27.3 and 28.4 mol%. Phylogenetic analyses indicated that the strains belong to Cluster I of the genus Clostridium (sensu Collins et al., 1994). The new strains differed from phylogenetically related clostridia in terms of cellular fatty acid composition, soluble protein profiles and phenotypic properties. On the basis of phenotypic and genotypic characterization data, the strains were assigned to a new species for which the name Clostridium frigidicarnis is proposed; strain SPL77AT (= DSM 12271T) is the type strain.
DOI：
10.1099/00207713-44-4-812
发表时间：
1994-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
COLLINS, MD;LAWSON, PA;FARROW, JAE
通讯作者：
FARROW, JAE