The Double-Stranded RNA Analog, Poly(I:C), Triggers Distinct Transcriptomic Shifts in Keratinocyte Subsets.
The Double-Stranded RNA Analog, Poly(I:C), Triggers Distinct Transcriptomic Shifts in Keratinocyte Subsets.
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DOI：
10.1016/j.jid.2022.03.015
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发表时间：
2022-10
影响因子：
6.5
通讯作者：
Nagao, K.
中科院分区：
文献类型：
--
作者：
Sakamoto, K.;Nagao, K.
病毒感染期间的 I 型干扰素 (IFN) 反应会激活宿主保护性免疫，但也可能引发红斑狼疮和银屑病的自身免疫级联反应（Illescas-Montes 等，2019；Wang 等，2021）。抗病毒免疫的各个方面可以通过腹膜内注射双链 RNA 类似物 Poly (I:C) 在小鼠中进行建模，它会激发全身 I 型 IFN 的产生 (Kühn et al., 1995)。利用这种方法，我们最近报道，上毛囊、漏斗部 (IFD) 和峡部 (ISM) 中的 ADAM10-Notch 信号通路增强了 I 型 IFN 反应期间的先天上皮屏障 (Sakamoto et al., 2021)。然而，I 型 IFN 反应期间角质形成细胞亚群的转录组学状况尚未得到系统探索，对此的理解可以加深我们对角质形成细胞如何促进炎症的认识。因此，我们对 C57BL/6 小鼠一次性腹腔注射 200 μg 聚 (I:C) 或生理盐水，制备表皮单细胞悬液 (Sakamoto et al., 2022)，并分选滤泡间表皮 (IFE) 和凸起 (图 1a) 以进行 RNA 测序。使用我们最近报告的 IFD 和 ISM、GSE18083 上的 RNA 测序数据对获得的数据进行分析（Sakamoto 等人，2021）。本研究中的 GSE180803 和 GSE180435 同时测序。所有实验程序均得到 NIAMS 动物护理和使用委员会的批准。利用 Partek® Flow® 进行数据处理和基因表达分析。用于分选的标记基因的表达水平表明角质形成细胞亚群已正确获得（图1b）。生成表达基因的维恩图
Type I interferons (IFN) responses during viral infections activate host-protective immunity, but it may also trigger autoimmune cascades in lupus erythematosus and psoriasis (Illescas-Montes et al., 2019, Wang et al., 2021). Aspects of antiviral immunity can be modeled in mice via intraperitoneal injection of the double-stranded RNA analog, poly (I: C), which provokes systemic type I IFN production (Kühn et al., 1995). Utilizing this approach, we recently reported that innate epithelial barrier during a type I IFN response was bolstered by ADAM10-Notch signaling pathway in upper hair follicles, infundibulum (IFD) and isthmus (ISM)(Sakamoto et al., 2021). However, the transcriptomic landscape of keratinocyte subsets during type I IFN responses has not been systematically explored, the understanding of which could deepen our insight on how keratinocytes contribute to inflammation. Thus, we subjected C57BL/6 mice to one-time intraperitoneal injection with 200 μg of poly (I: C) or saline, prepared epidermal singlecell suspensions (Sakamoto et al., 2022), and sorted interfollicular epidermis (IFE) and the bulge (Figure 1a) to perform RNA-sequencing. Obtained data were analyzed with our recently reported RNA-sequencing data on the IFD and ISM, GSE18083 (Sakamoto et al., 2021). GSE180803 and GSE180435 from this study were sequenced simultaneously. All experiments procedures were approved by the NIAMS Animal Care and Use Committee. Data processing and gene expression analyses were performed utilizing Partek® Flow®. Expression levels of marker genes used for sorting revealed that keratinocyte subsets were properly acquired (Figure 1b). Generation of a Venn diagram for genes expressed at
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影响因子：
32.4
作者：
Sakamoto K;Jin SP;Goel S;Jo JH;Voisin B;Kim D;Nadella V;Liang H;Kobayashi T;Huang X;Deming C;Horiuchi K;Segre JA;Kong HH;Nagao K
通讯作者：
Nagao K
干扰素调节因子介导的信号传导在银屑病中的作用
影响因子：
3.6
作者：
Wang WM;Li F;Jin HZ
通讯作者：
Jin HZ
影响因子：
5.4
作者：
Rasmussen, Simon B.;Sorensen, Louise N.;Paludan, Soren R.
通讯作者：
Paludan, Soren R.
影响因子：
11.8
作者：
Gonzales KAU;Fuchs E
通讯作者：
Fuchs E
影响因子：
4.4
作者：
Tsoi, Lam C.;Hile, Grace A.;Kahlenberg, J. Michelle
通讯作者：
Kahlenberg, J. Michelle